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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 3 antibody [Calp3d/2C4]
See all Calpain 3 products (13) ...
Description
Mouse monoclonal [Calp3d/2C4] to Calpain 3
Specificity
ab51861 reacts with full-size calpain 3 (94kD) plus an additional fragment at 30kD in human skeletal muscle. A band of 94kD is seen with rabbit and dog muscle while extracts of hamster muscle show reactivity with the 94kD band and a larger species of approximately 110kD. This 110kD band is the principal immunoreactive species seen in rat muscle extracts.
Tested applications
Cross reactivity
Reacts with
Rabbit, Hamster, Dog, Human
Predicted to work with
Rat, Sheep, Cow
Does not react with
Mouse, Chicken, Pig
Immunogen
Synthetic peptide: MPTVISASVAPRTAAEPRS, corresponding to amino acids 1-19 of Human Calpain 3
MPTVISASVA PRTAAEPRS
Positive control
Western Blotting - Freshly isolated rat or normal human skeletal muscle homogenate prepared in treatment buffer containing 0.125M tris-HCI buffer pH6.4, 10% glycerol, 4% SDS, 4M urea, 10% mercaptoethanol and 0.001% bromophenol blue (final pH of treatment buffer, 6.8). Staining pattern Bands of approximately 94, 80 and 60kD in rat muscle extracts and/or bands of approximately 78 and 94kD in Western blots of human muscle extracts.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
Calp3d/2C4
Myeloma
x63-Ag8.653
Isotype
IgG2b
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/25 - 1/50. Predicted molecular weight: 94 kDa.
The optimal dilution should be determined by the user as calpain must be homogenised in treatment buffer (see Positive Controls) immediately after harvest to prevent degradation.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Calcium-regulated non-lysosomal thiol-protease.
Tissue specificity
Isoform I is skeletal muscle specific.
Involvement in disease
Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP20807
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium-activated neutral proteinase 3 antibody
- Calpain L3 antibody
- Calpain large polypeptide L3 antibody
see all
Database links
- Entrez Gene: 825 Human
- Entrez Gene: 29155 Rat
- Omim: 114240 Human
- SwissProt: P20807 Human
- SwissProt: P16259 Rat
- Unigene: 143261 Human
- Unigene: 9726 Rat
References for Anti-Calpain 3 antibody [Calp3d/2C4] (ab51861)
ab51861 has not yet been referenced specifically in any publications.
Publishing research using ab51861? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"