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Anti-Calpain 3 antibody - Domain I, large subunit (ab38960)

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Overview

Product name

Anti-Calpain 3 antibody - Domain I, large subunit
See all Calpain 3 products (13) ...

Description

Rabbit polyclonal to Calpain 3 - Domain I, large subunit

Specificity

Ab38960 binds to Calpain 3 (reduced and non reduced), and recognizes both the latent and active forms of the protein. It does not cross react with the other Calpain family members (m calpain, M calpain, LP-82/85 Calpain, ncl2, ncl3, etc.). The rat 88 and 90 kD novel Calpains share the insert #1, and ab38960 may crossreact with these forms.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide based on domain I of human Calpain 3 large subunit.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects bands of approximately 94, 82, 62 and 60 kDa and a series of further cleaved active forms when used against the reduced protein (predicted molecular weight of the latent form: 94 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Calcium-regulated non-lysosomal thiol-protease.

Tissue specificity

Isoform I is skeletal muscle specific.

Involvement in disease

Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.

Sequence similarities

Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP20807 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Calcium activated neutral proteinase 3 antibody
  • Calcium-activated neutral proteinase 3 antibody
  • Calpain L3 antibody
  • Calpain large polypeptide L3 antibody
  • Calpain p94 antibody
  • Calpain-3 antibody
  • CAN3_HUMAN antibody
  • CANP 3 antibody
  • CANP3 antibody
  • CANPL3 antibody
  • CAPN 3 antibody
  • CAPN3 antibody
  • LGMD 2A antibody
  • LGMD2 antibody
  • LGMD2A antibody
  • Limb girdle muscular dystrophy 2A antibody
  • Lp82 antibody
  • LP85 antibody
  • Muscle specific calcium activated neutral protease 3 antibody
  • Muscle-specific calcium-activated neutral protease 3 antibody
  • nCL-1 antibody
  • nCL1 antibody
  • New calpain 1 antibody
  • p94 antibody
see all

Database links

see all

Protocols

  • Recommended WB protocol

    • References for Anti-Calpain 3 antibody - Domain I, large subunit (ab38960)

    This product has been referenced in:

    • Ono Yet al. Skeletal muscle-specific calpain is an intracellular Na+-dependent protease. J Biol Chem 285:22986-98 (2010). WB; African Green Monkey.Read more (PubMed: 20460380) »

    See 1 publication for this product

    Publishing research using ab38960? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"