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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Anti-Calpain 3 antibody - Domain II (catalytic domain), large subunit (ab38962)
Overview
Product name
Anti-Calpain 3 antibody - Domain II (catalytic domain), large subunit
See all Calpain 3 products (13) ...
Description
Rabbit polyclonal to Calpain 3 - Domain II (catalytic domain), large subunit
Specificity
The antibody does not cross react with the other calpain family members (m-calpain, M-calpain, LP-82/85 Calpain, ncl-2, ncl-3, etc.). The antibody may crossreact with rat 88 and 90 kD novel Calpains due to Domain II sequence similarities.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide based on Insert 1, in domain-II of human Calpain 3 large subunit. (Peptide available as ab41306.)
General notes
EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen (94 kDa form).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Western blot - Calpain 3 antibody - Domain II (catalytic domain), large subunit (ab38962)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein, Ab38962 identifies bands at 94 Kd, 82 Kd, 62 Kd, 60 Kd, and a series of further cleaved active forms. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Calcium-regulated non-lysosomal thiol-protease.
Tissue specificity
Isoform I is skeletal muscle specific.
Involvement in disease
Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP20807
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium activated neutral proteinase 3 antibody
- Calcium activated neutral proteinase 3 antibody
- Calcium-activated neutral proteinase 3 antibody
see all
Database links
- Entrez Gene: 825 Human
- Entrez Gene: 12335 Mouse
- Entrez Gene: 29155 Rat
- Omim: 114240 Human
- SwissProt: P20807 Human
- SwissProt: Q64691 Mouse
- SwissProt: P16259 Rat
- Unigene: 143261 Human
see all
Anti-Calpain 3 antibody - Domain II (catalytic domain), large subunit images:
Western blot - Calpain 3 antibody - Domain II (catalytic domain), large subunit (ab38962)
All lanes : Anti-Calpain 3 antibody - Domain II (catalytic domain), large subunit (ab38962) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 94 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Calpain 3 antibody - Domain II (catalytic domain), large subunit (ab38962)
This product has been referenced in:
- Moretti Det al. Novel variants of muscle calpain 3 identified in human melanoma cells: cisplatin-induced changes in vitro and differential expression in melanocytic lesions. Carcinogenesis 30:960-7 (2009).Read more (PubMed: 19386580) »
See 1 publication for this product
Publishing research using ab38962? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"