Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 3 antibody - Domain IV
See all Calpain 3 products (13) ...
Description
Rabbit polyclonal to Calpain 3 - Domain IV
Specificity
The antibody binds to Calpain 3, and recognizes both the latent and active forms. It does not cross react with the other Calpain family members (LP82 calpain, Mu calpain, M calpain, ncl2, ncl3, etc.).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide based on a unique insert found in the EF hand (the calcium binding domain IV of LP85 calpain large subunit) of Rat Calpain 3. (Peptide available as ab41308.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide affinity purified and concentrated.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. When used against the reduced protein, this antibody identifies bands at 85 kDa (the latent large subunit), 62 kDa (the amino terminal truncation at activation), and a series of further cleaved active forms. A smaller band at about 58 kDa is seen in human tissue samples; this may be a closely related species. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Calcium-regulated non-lysosomal thiol-protease.
Tissue specificity
Isoform I is skeletal muscle specific.
Involvement in disease
Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP20807
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium activated neutral proteinase 3 antibody
- Calcium-activated neutral proteinase 3 antibody
- Calpain L3 antibody
see all
Database links
- Entrez Gene: 12335 Mouse
- Entrez Gene: 29155 Rat
- SwissProt: Q64691 Mouse
- SwissProt: P16259 Rat
- Unigene: 396539 Mouse
- Unigene: 458021 Mouse
- Unigene: 9726 Rat
Protocols
References for Anti-Calpain 3 antibody - Domain IV (ab39878)
ab39878 has not yet been referenced specifically in any publications.
Publishing research using ab39878? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"