Calpain 3 peptide (Domain II) (ab41306)

Product Name 

Calpain 3 peptide (Domain II)

Product type 

Proteins and Peptides

Peptide 

This peptide was used as an immunogen for ab38962 - Calpain 3 antibody - Domain II (catalytic domain) and large subunit.

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 3 antibodies

Tested applications 
(see key)

ELISA, WB

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab38962. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Relevance 

Calpain 3, also known as calpain 94 or muscle calpain, is an intracellular, calcium dependent cysteine protease. The calpains have papain like activity, thus the pain nomenclature. The calpain family members consist of a common small subunit, and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. Unlike M calpain which is ubiquitously expressed, Calpain 3 is a member of the tissue specific calpains. Unlike the other calpains, the endogenous calpain inhibitor, calpastatin, does not seem to inhibit Calpain 3. The half life of native Calpain 3 is also much shorter; about 10 minutes. Mutations in Calpain 3 have been linked to Limb Girdle Muscular Dystrophy, and native Calpain 3 is known to act as a meat tenderizer. The latent large subunit is 94 kDa, and the amino terminal truncation at activation yields an approximately 64 to 62 kDa form. A cascade of smaller forms can be seen with further activation.

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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