Calpain 5 peptide (Domain II) (ab41311)

Product Name 

Calpain 5 peptide (Domain II)

Product type 

Proteins and Peptides

Peptide 

This peptide was used as an immunogen for ab38943 - Calpain 5 antibody - Domain II (catalytic domain) and large subunit.

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 5 antibodies

Tested applications 
(see key)

ELISA, WB

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab38943. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Relevance 

Calpain proteinases are defined currently as papain like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date are not known to require a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV (Domain T in calpains 5 & 6).

Calpain 5, also known as the human orthologue of TRA3, a C. elegans protein involved in sex determination, is located on chromosome 11 in humans, near calpain 1. Calpain 5 is an intracellular, calcium dependent cysteine protease. Calpain 5 domain T does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. It is also unclear if calpain 5 plays any role in mammalian sex determination, since the enzyme is found in colon, kidney, liver and trachea and other tissues, as well as testis.

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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