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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 6 antibody - Carboxyterminal end domain-T
See all Calpain 6 products (4) ...
Description
Rabbit polyclonal to Calpain 6 - Carboxyterminal end domain-T
Specificity
ab38938 binds to Calpain 6, but does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 2, ncl 3, etc.). It reacts to Domain-T of Calpain 6, and detects both latent and aminoprocessed (activated) Calpain 6.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to the carboxyterminal end of the large subunit of Human Calpain 6. (Peptide available as ab41315.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 75 KD, 58 KD, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues.
Tissue specificity
Expressed only in placenta.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 C2 domain.
Contains 1 calpain catalytic domain.
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle.
Target information above from: UniProt accessionQ9Y6Q1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CALP M antibody
- Calpain-6 antibody
- Calpain-like protease X-linked antibody
see all
Database links
- Entrez Gene: 827 Human
- Entrez Gene: 12338 Mouse
- Entrez Gene: 83685 Rat
- Omim: 300146 Human
- SwissProt: Q9Y6Q1 Human
- SwissProt: O35646 Mouse
- SwissProt: O88501 Rat
- Unigene: 496593 Human
see all
Protocols
References for Anti-Calpain 6 antibody - Carboxyterminal end domain-T (ab38938)
ab38938 has not yet been referenced specifically in any publications.
Publishing research using ab38938? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"