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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Anti-Calpain 6 antibody - Domain II (catalytic domain), large subunit (ab38939)
Overview
Product name
Anti-Calpain 6 antibody - Domain II (catalytic domain), large subunit
See all Calpain 6 products (4) ...
Description
Rabbit polyclonal to Calpain 6 - Domain II (catalytic domain), large subunit
Specificity
ab38939 binds to the Domain-II, large subunit of Calpain 6, but does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 2, ncl 3, etc.).
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to the domain-II in the large subunit of Human Calpain 6. (Peptide available as ab41314.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).(When used against the reduced protein this antibody identifies bands at 75 kD, 58kD, and a series of further cleaved active forms. )
ICC/IF: Use at an assay dependent concentration. (PubMed: 21406564)
Target
Function
Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues.
Tissue specificity
Expressed only in placenta.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 C2 domain.
Contains 1 calpain catalytic domain.
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle.
Target information above from: UniProt accessionQ9Y6Q1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CALP M antibody
- Calpain-6 antibody
- Calpain-like protease X-linked antibody
see all
Database links
- Entrez Gene: 827 Human
- Entrez Gene: 12338 Mouse
- Entrez Gene: 83685 Rat
- Omim: 300146 Human
- SwissProt: Q9Y6Q1 Human
- SwissProt: O35646 Mouse
- SwissProt: O88501 Rat
- Unigene: 496593 Human
see all
Protocols
References for Anti-Calpain 6 antibody - Domain II (catalytic domain), large subunit (ab38939)
This product has been referenced in:
- Tonami Ket al. Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1. J Cell Sci 124:1214-23 (2011). ICC/IF; Mouse.Read more (PubMed: 21406564) »
See 1 publication for this product
Publishing research using ab38939? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"