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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 6 antibody
See all Calpain 6 products (4) ...
Description
Rabbit polyclonal to Calpain 6
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
An 18 amino acid peptide from near the carboxy terminus of human Calpain 6.
Positive control
Rat lung tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity chromatography purified via peptide column.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Predicted molecular weight: 75 kDa.
IHC-P: Use a concentration of 2.5 µg/ml
Target
Function
Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues.
Tissue specificity
Expressed only in placenta.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 C2 domain.
Contains 1 calpain catalytic domain.
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle.
Target information above from: UniProt accessionQ9Y6Q1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CALP M antibody
- Calpain-6 antibody
- Calpain-like protease X-linked antibody
see all
Database links
- Entrez Gene: 827 Human
- Entrez Gene: 12338 Mouse
- Entrez Gene: 83685 Rat
- Omim: 300146 Human
- SwissProt: Q9Y6Q1 Human
- SwissProt: O35646 Mouse
- SwissProt: O88501 Rat
- Unigene: 496593 Human
see all
Anti-Calpain 6 antibody images:
Western blot - Calpain 6 antibody (ab76974)
Lane 1 : Anti-Calpain 6 antibody (ab76974) at 0.5 µg/ml
Lane 2 : Anti-Calpain 6 antibody (ab76974) at 1 µg/ml
Lane 1 : rat lung tissue lysate
Lane 2 : rat lung tissue lysate
Predicted band size : 75 kDa
Observed band size : 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calpain 6 antibody (ab76974)
ab76974 at 2.5µg/ml staining Calpain6 in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
References for Anti-Calpain 6 antibody (ab76974)
ab76974 has not yet been referenced specifically in any publications.
Publishing research using ab76974? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"