Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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I would like to know which is a better antibody to use ab38937 or ab28212 for WB? I have used a N-terminal anti-calpain successfully in the past and am concerned the recommended c-terminus anti-calpain is not as good. |
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ANSWER: |
Thanks for your inquiry. Calpains are cleared at the aminoterminal end, so sometimes the amino-specific Antibodies miss the cleaved forms. Either will work for calpain-7, but using the carboxy-specific antibody will let them see both forms. I hope this answers your question. Please feel free to contact me if you require further assistance. |
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Predicted band size : 93 kDa
Proteins were extracted from the various strata of the lens using a progressive lysis technique which, in this example, generated seven fractions, from the outer cell layers (F1) to the lens core (F7). Calpain 1, 2, and 7 are readily detected in the outer cell layers, but their abundance decreases with depth, and these calpains are not detected in the innermost (F7) fiber cells. In contrast, calpain 3 is not detected in the outermost cells, first appearing in the cortical (F2) fraction. Calpain 3 signal is maximal in F3-F4 and persists in the innermost (F7) fraction.Calpain 1 : ab39170.Calpain 2 : ab39165.Calpain 3 : Non Abcam antibody.Calpain 7 : ab38937.Primary antibodies were detected using an horseradish peroxidase-conjugated secondary antibody and chemiluminescence.
Image from De Maria A et al, J Biol Chem. 2009 May 15;284(20):13542-50. Epub 2009 Mar 6, Fig 3. DOI 10.1074/jbc.M900561200
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