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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Calpain 8 peptide (Aminoterminal end domain I)
See all Calpain 8 products (2) ...
Protein description
This peptide was used as an immunogen for ab28214 - Calpain 8 antibody - Aminoterminal end domain I.
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
Concentration
Concentration information loading...
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab28214. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
Calpain proteinases are defined currently as papain like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date are not known to require a small subunit, although there is evidence that the calpain small subunit #2 may act as a chaperonin in the folding of calpains, and then disassociate. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV (Domain T in calpains 5 & 6). Calpain 8, also known as NCL 2 (novel calpain 2), was originally characterized in rodents as a stomach specific protein, but the human sequence was isolated from leukocytes. Most similar to calpain 2 (60 % identical to rat or human sequences), murine calpain 8 is also known as a truncated form (Calpain NCL-2), lacking the calcium binding domain, and most of domain III. Homology between human calpain 8 and human calpain 9 (the human stomach specific calpain) is much lower; calpain 9 is more similar to calpain 3. Homology between rat, mouse and human calpain 8 is high. The large subunit of calpain 8 zymogen runs at approximately 80 Kd, and the amino terminal truncation at activation yields an approximately 60 kD form. Cleavage of the carboxyterminal region generates smaller forms of Calpain 8, but it is not clear if these forms are proteolytically active.
Cellular localization
Intracellular
References for Calpain 8 peptide (Aminoterminal end domain I) (ab41353)
ab41353 has not yet been referenced specifically in any publications.
Publishing research using ab41353? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"