Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 9 antibody - Domain II (catalytic domain), large subunit
See all Calpain 9 products (8) ...
Description
Rabbit polyclonal to Calpain 9 - Domain II (catalytic domain), large subunit
Specificity
The antibody recognizes latent and aminotruncated (active) Calpain 9, and binds to both the reduced and non-reduced protein. It does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on domain II (catalytic domain) in the large subunit of Human Calpain 9. (Peptide available as ab41357.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purification notes
Ab38957 has been peptide affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein, Ab38957 identifies bands at 80 Kd, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Calcium-regulated non-lysosomal thiol-protease.
Tissue specificity
Expressed predominantly in stomach.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains.
Target information above from: UniProt accessionO14815
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calpain-9 antibody
- Calpain9 antibody
- CAN9_HUMAN antibody
see all
Database links
- Entrez Gene: 10753 Human
- Entrez Gene: 73647 Mouse
- Entrez Gene: 116694 Rat
- Omim: 606401 Human
- SwissProt: O14815 Human
- SwissProt: Q6PIV8 Human
- SwissProt: Q6PDE6 Mouse
- SwissProt: Q9D805 Mouse
see all
Protocols
References for Anti-Calpain 9 antibody - Domain II (catalytic domain), large subunit (ab38957)
ab38957 has not yet been referenced specifically in any publications.
Publishing research using ab38957? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"