Calpain 9 antibody - Domain IV (EF-1), large subunit (ab28218)

Product Name 

Calpain 9 antibody - Domain IV (EF-1), large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 9 - Domain IV (EF-1), large subunit

Immunogen 

Synthetic peptide (Human) based on domain-IV in the large subunit.(Peptide available as ab41358.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody recognizes latent and aminotruncated (active) calpain 9, but not the truncated form lacking the domain-IV. It does not cross react with the other calpain family members (m-calpain, calpain-94, ncl-2, ncl-3, etc.)

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 9 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 80 kDa and a series of bands due to cleaved forms, (under reducing conditions). (predicted molecular weight: 79 kDa). Notes: a recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Relevance 

Calpain 9 is an intracellular, calcium dependent cysteine protease. Calpain 9 was first described in the digestive tract, and a shorter form with a deletion in the catalytic domain has been associated with gastric cancer. Recombinant calpain 9 has been expressed, and is unstable unless expressed with the calpain small subunit (calpain S1), suggesting that calpain 9 associates with the small subunit in vivo. The recombinant calpain 9 showed caseinolytic activity, albeit at a lower level than native calpain 1 or calpain 2, and a calcium sensitivity intermediate between calpain 1 and calpain 2. Calpastatin blocked the caseinolytic activity. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The latent large subunit migrates at about 79 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 kD form. A cascade of smaller forms can be seen with further activation. It is expressed predominantly in the stomach although a shorter form with a deletion in the catalytic domain has been associated with gastric cancer.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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