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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Calpain S1 antibody - Carboxyterminal end domain V
See all Calpain S1 products (13) ...
Description
Rabbit polyclonal to Calpain S1 - Carboxyterminal end domain V
Specificity
This antibody binds to calpain S1, and recognizes latent and amino processed forms. It does not cross react with the other calpain family members (calpain S2, calpain 1, calpain 2, calpain 3, etc.)
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) based on the carboxyterminal end of domain V of the small subunit. (Peptide available as ab41356.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects bands of approximately 30kDa and 28kDa (amino truncated form) under reducing conditions. (Predicted molecular weight: 30 kDa). Calpain S1 is quickly degraded, hence samples must be lysed in the presence of protease inhibiters to maintain latency. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.
Cellular localization
Cytoplasmic; translocates to the plasma membrane upon calcium binding
Alternative names
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium Activated Neutral Proteinase Small Subunit antibody
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Database links
- Entrez Gene: 826 Human
- Entrez Gene: 12336 Mouse
- Entrez Gene: 29156 Rat
- Omim: 114170 Human
- SwissProt: P04632 Human
- SwissProt: O88456 Mouse
- SwissProt: Q64537 Rat
- Unigene: 515371 Human
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Protocols
References for Anti-Calpain S1 antibody - Carboxyterminal end domain V (ab28217)
ab28217 has not yet been referenced specifically in any publications.
Publishing research using ab28217? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"