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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain S1 antibody - Carboxyterminal end domain VI(ab28240)
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Product Name
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Calpain S1 antibody - Carboxyterminal end domain VI
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See all Calpain S1 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Calpain S1 - Carboxyterminal end domain VI
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Immunogen
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Synthetic peptide based on the carboxyterminal end of domain-VI of the small subunit, using the human Calpain S1 sequence.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody is specific for Calpain S1. It does not cross react with the other calpain family members (calpain S-2, calpain-1, calpain-2, calpain-3, etc...)
We have a range of domain specific antibodies for this target. For a full list please see all Calpain S1 antibodies
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 30kDa and 28kDa (predicted molecular weight: 30 kDa). Calpain S1 is quickly degraded, and samples must be lysed in the presence of protease inhibiters to maintain latency. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic; translocates to the plasma membrane upon calcium binding
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain S1 antibody - Carboxyterminal end domain VI is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Calpain S1 antibody - Carboxyterminal end domain VI images, references, products related to ab28240 and other tools.
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Calpain S1 antibody - Carboxyterminal end domain VI images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain S1 antibody - Carboxyterminal end domain VI(ab28240)
Ab28240 staining Human normal skeletal muscle. Staining is localized to cytoplasmic compartment.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for Calpain S1 antibody - Carboxyterminal end domain VI (ab28240)
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This product has been used in:
Kellmann R et al. Proteomic response of human neuroblastoma cells to azaspiracid-1. J Proteomics 72:695-707 (2009). WB, ICC/IF; Human. PubMed: 19269354
If you publish research using ab28240 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Calpain S1
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Calpain S1 antibody - Carboxyterminal end domain VI - more information
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