|
|
|
|
IHC-P - Calpain S1 antibody (ab54929)
(enlarge)
|
|
Western blot - Calpain S1 antibody (ab54929)
(enlarge)
|
|
|
|
|
Product Name
|
Calpain S1 antibody
|
| |
See all Calpain S1 antibodies (12)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal to Calpain S1
|
|
Immunogen
|
Recombinant fragment: KRWQAIYKQF DTDRSGTICS SELPGAFEAA GFHLNEHLYN MIIRRYSDES GNMDFDNFIS CLVRLDAMFR AFKSLDKDGT GQIQVNIQE, corresponding to amino acids 172-260 of Human Calpain S1
BLAST 'KRWQAIYKQF DTDRSGTICS SELPGAFEAA GFHLNEHLYN MIIRRYSDES GNMDFDNFIS CLVRLDAMFR AFKSLDKDGT ' with  or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic; translocates to the plasma membrane upon calcium binding
|
|
Research areas
|
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids
|
|
Relevance
|
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: None
PBS, pH 7.2
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain S1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
Calpain S1 antibody images:
|
 IHC-P - Calpain S1 antibody (ab54929)
Calpain S1 antibody (ab54929) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
|
| |
Western blot - Calpain S1 antibody (ab54929)
Predicted band size : 28 kDa
Calpain S1 antibody (ab54929) at 1ug/lane + A-431 cell lysate at 25ug/lane.
|
| |
References for Calpain S1 antibody (ab54929)
|
|
This product has been used in: (two most recent references)
McHugh BJ et al. Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum. J Cell Sci 123:51-61 (2010). WB; Human. PubMed: 20016066
Wiesen JL & Tomasi TB Dicer is regulated by cellular stresses and interferons. Mol Immunol 46:1222-8 (2009). PubMed: 19118902
If you publish research using ab54929 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Calpain S1
|
| |
Calpain S1 antibody - more information
|
Customer reviews (feedback) regarding Calpain S1 antibody |
Customer FAQs regarding Calpain S1 antibody |
Protocols for Calpain S1 antibody |
Price and availability of products related to Calpain S1 antibody |
| |
Calpain S1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Calpain S1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
