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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Calpain S1 antibody
See all Calpain S1 products (13) ...
Description
Mouse monoclonal to Calpain S1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: KRWQAIYKQF DTDRSGTICS SELPGAFEAA GFHLNEHLYN MIIRRYSDES GNMDFDNFIS CLVRLDAMFR AFKSLDKDGT GQIQVNIQE, corresponding to amino acids 172-260 of Human Calpain S1
KRWQAIYKQF DTDRSGTICS SELPGAFEAA GFHLNEHLYN MIIRRYSDES GNMDFDNFIS CLVRLDAMFR AFKSLDKDGT GQIQVNIQE
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.
Cellular localization
Cytoplasmic; translocates to the plasma membrane upon calcium binding
Alternative names
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
Database links
- Entrez Gene: 826 Human
- Omim: 114170 Human
- SwissProt: P04632 Human
- Unigene: 515371 Human
Anti-Calpain S1 antibody images:
IHC-P - Calpain S1 antibody (ab54929)
Calpain S1 antibody (ab54929) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
Western blot - Calpain S1 antibody (ab54929)
Calpain S1 antibody (ab54929) at 1ug/lane + A-431 cell lysate at 25ug/lane.
References for Anti-Calpain S1 antibody (ab54929)
This product has been referenced in:
- McHugh BJet al. Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum. J Cell Sci 123:51-61 (2010). WB; Human.Read more (PubMed: 20016066) »
- Wiesen JL & Tomasi TB Dicer is regulated by cellular stresses and interferons. Mol Immunol 46:1222-8 (2009).Read more (PubMed: 19118902) »
See all 2 publications for this product
Publishing research using ab54929? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"