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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain S2 antibody - Aminoterminal end domain V
See all Calpain S2 products (3) ...
Description
Rabbit polyclonal to Calpain S2 - Aminoterminal end domain V
Specificity
The antibody is specific for Calpain S2, but does not cross react with the other calpain family members.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on the amino terminal end of domain-V of the small subunit, using the human Calpain S2 sequence.
(Peptide available as ab41378.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Immunocytochemistry/ Immunofluorescence - Anti-Calpain S2 antibody - Aminoterminal end domain V (ab28241)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 29 kDa (predicted molecular weight: 28 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Relevance
Both M-calpain and m-calpain are ubiquitously expressed in mammalian cells, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-3, calpain-5, calpain-9, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-S1), and a large variable subunit. It is not clear that all calpains form heterodimers with a small subunit, but calpain-S1 is known to form a heterodimer with calpain-1 and also with calpain-2. Calpain S2 was recently discovered, and initial studies indicate that both small subunits may act as chaperonins to help with folding, or accessory proteins to help localize the calpains. Domains in the small subunit include the amino terminal domain-V (a glycine-rich sequence that is removed on activation of calpain, shorter in calpain-S2 than in calpain S1) and domain-VI, the calcium binding, EF hand domain.
Cellular localization
Cytoplasmic; Translocates to the plasma membrane upon calcium binding
Alternative names
- Calcium dependent protease small subunit 2 antibody
- Calpain Short Chain 2 antibody
- Calpain Small 2 antibody
see all
Database links
- Entrez Gene: 84290 Human
- Entrez Gene: 69543 Mouse
- SwissProt: Q96L46 Human
- SwissProt: Q9D7J7 Mouse
- Unigene: 534503 Human
Anti-Calpain S2 antibody - Aminoterminal end domain V images:
Immunocytochemistry/ Immunofluorescence - Anti-Calpain S2 antibody - Aminoterminal end domain V (ab28241)
ICC/IF image of ab28481 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28481, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Calpain S2 antibody - Aminoterminal end domain V (ab28241)
ab28241 has not yet been referenced specifically in any publications.
Publishing research using ab28241? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"