Calpain S2 antibody (ab24006)

Product Name 

Calpain S2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain S2

Immunogen 

Synthetic peptide based on the carboxyterminal end of domain-VI (the calcium binding, EF hand domain) of Calpain S2 (Human).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab24006 binds to calpain S2, but does not cross react with the other calpain family members. It recognises latent and amino-processed calpain S1; the latent small subunit is 30 Kd, and the amino terminal truncation at activation yields an approximately 28 kD form.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Predicted molecular weight: 28 kDa. Calpain S2 is quickly degraded and samples must be lysed in the presence of protease inhibiters to maintain latency. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

ab24006 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic; Translocates to the plasma membrane upon calcium binding

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Relevance 

Both M-calpain and m-calpain are ubiquitously expressed in mammalian cells, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-3, calpain-5, calpain-9, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-S1), and a large variable subunit. It is not clear that all calpains form heterodimers with a small subunit, but calpain-S1 is known to form a heterodimer with calpain-1 and also with calpain-2. Calpain S2 was recently discovered, and initial studies indicate that both small subunits may act as chaperonins to help with folding, or accessory proteins to help localize the calpains. Domains in the small subunit include the amino terminal domain-V (a glycine-rich sequence that is removed on activation of calpain, shorter in calpain-S2 than in calpain S1) and domain-VI, the calcium binding, EF hand domain.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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