You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Calpastatin antibody [2G11D6] (ab3515)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Calpastatin antibody [2G11D6]
See all Calpastatin products (11) ...

Description

Mouse monoclonal [2G11D6] to Calpastatin

Specificity

Detects Calpastatin. This antibody does not cross-react with calpains or calmodulin.

Tested applications

ICC, WBmore details

Cross reactivity

Reacts with

Rat, Cow, Dog, Human, Pig

Immunogen

Bovine skeletal muscle Calpastatin.

Epitope

This antibody recognizes an epitope found between amino acid residues 295-501 (domain II) of human Calpastatin.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Ascites

Primary antibody notes

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When Calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Clonality

Monoclonal

Clone number

2G11D6

Isotype

IgG2a

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ICC

     
  • WB

     

Application notes

Immunocytochemistry: 1/1000.
Western Blot: 1/5000.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene.

Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells results in diffuse cytoplasmic staining and weak nuclear staining.

Target

Function

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

Sequence similarities

Belongs to the protease inhibitor I27 (calpastatin) family.

Domain

Has four inhibitory domains.

Post-translational
modifications

The N-terminus is blocked.

Target information above from: UniProt accessionP20810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BS 17 antibody
  • Calpain inhibitor antibody
  • Calpastatin antibody
  • CAST antibody
  • Heart type calpastatin antibody
  • ICAL_HUMAN antibody
  • MGC9402 antibody
  • Sperm BS 17 component antibody
  • Sperm BS-17 component antibody
see all

References for Anti-Calpastatin antibody [2G11D6] (ab3515)

This product has been referenced in:

  • Liang Bet al. Calpain Activation Promotes BACE1 Expression, Amyloid Precursor Protein Processing, and Amyloid Plaque Formation in a Transgenic Mouse Model of Alzheimer Disease. J Biol Chem 285:27737-44 (2010). WB; Mouse.Read more (PubMed: 20595388) »
  • Forhead AJet al. Role of leptin in the regulation of growth and carbohydrate metabolism in the ovine fetus during late gestation. J Physiol 586:2393-403 (2008).Read more (PubMed: 18325979) »

See all 2 publications for this product

Publishing research using ab3515? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"