Calpastatin antibody [2G11D6] (ab3515)

Product Name 

Calpastatin antibody [2G11D6]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2G11D6] to Calpastatin

Immunogen 

Bovine skeletal muscle Calpastatin.

Reacts with 
(species key)

Hu, Rat, Cow, Dog, Pig

Specificity 

Detects Calpastatin.
This antibody does not cross-react with calpains or calmodulin.

Epitope 

This antibody recognizes an epitope found between amino acid residues 295-501 (domain II) of human Calpastatin.

Tested applications 
(see key)

ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Immunocytochemistry: 1/1000.
Western Blot: 1/5000.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene.

Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells results in diffuse cytoplasmic staining and weak nuclear staining.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.

Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When Calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2G11D6

Isotype 

IgG2a

Purity 

Ascites

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Calpastatin antibody [2G11D6] (ab3515)

  This product has been used in:

Forhead AJ et al. Role of leptin in the regulation of growth and carbohydrate metabolism in the ovine fetus during late gestation. J Physiol 586:2393-403 (2008). PubMed: 18325979

If you publish research using ab3515 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Calpastatin antibody [2G11D6]

Customer FAQs regarding Calpastatin antibody [2G11D6]

Protocols for Calpastatin antibody [2G11D6]

Price and availability of products related to Calpastatin antibody [2G11D6]

Calpastatin antibody [2G11D6] - related products: