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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Calpastatin antibody [2G11D6]
See all Calpastatin products (11) ...
Description
Mouse monoclonal [2G11D6] to Calpastatin
Specificity
Detects Calpastatin. This antibody does not cross-react with calpains or calmodulin.
Tested applications
ICC, WBmore details
Cross reactivity
Reacts with
Rat, Cow, Dog, Human, Pig
Immunogen
Bovine skeletal muscle Calpastatin.
Epitope
This antibody recognizes an epitope found between amino acid residues 295-501 (domain II) of human Calpastatin.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Ascites
Primary antibody notes
The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When Calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.
Clonality
Monoclonal
Clone number
2G11D6
Isotype
IgG2a
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
WB
Application notes
Immunocytochemistry: 1/1000.
Western Blot: 1/5000.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene.
Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells results in diffuse cytoplasmic staining and weak nuclear staining.
Target
Function
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Sequence similarities
Belongs to the protease inhibitor I27 (calpastatin) family.
Domain
Has four inhibitory domains.
Post-translational
modifications
The N-terminus is blocked.
Target information above from: UniProt accessionP20810
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BS 17 antibody
- Calpain inhibitor antibody
- Calpastatin antibody
see all
Database links
- Entrez Gene: 281039 Cow
- Entrez Gene: 831 Human
- Entrez Gene: 25403 Rat
- Omim: 114090 Human
- SwissProt: P20811 Cow
- SwissProt: P20810 Human
- SwissProt: P12675 Pig
- SwissProt: P27321 Rat
see all
References for Anti-Calpastatin antibody [2G11D6] (ab3515)
This product has been referenced in:
- Liang Bet al. Calpain Activation Promotes BACE1 Expression, Amyloid Precursor Protein Processing, and Amyloid Plaque Formation in a Transgenic Mouse Model of Alzheimer Disease. J Biol Chem 285:27737-44 (2010). WB; Mouse.Read more (PubMed: 20595388) »
- Forhead AJet al. Role of leptin in the regulation of growth and carbohydrate metabolism in the ovine fetus during late gestation. J Physiol 586:2393-403 (2008).Read more (PubMed: 18325979) »
See all 2 publications for this product
Publishing research using ab3515? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"