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Anti-Calpastatin antibody - Aminoterminal end (Domain L) Testis Isoform (ab28252)

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1 question for ab28252

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Question 1

Wednesday 30-August-2006

One of our customers wants to order ab28252 or ab28253. The tested applications of them are IHC, IP, WB etc in your on-line datasheets. I want to know if they could be applied to IHC-Paraffin.

ANSWER:

 

Thank you for your enquiry.

Yes, the antibodies can be used in IHC-P and I have updated the datasheet accordingly. Please use them at an assay dependent dilution using heat-mediated antigen retrieval in citrate buffer pH 6.0. I would recommend a starting dilution of 1:100 although this dilution may have to be optimized.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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