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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Calpastatin antibody - Aminoterminal end (Domain L) Testis Isoform
See all Calpastatin products (11) ...
Description
Rabbit polyclonal to Calpastatin - Aminoterminal end (Domain L) Testis Isoform
Specificity
ab28252 recognises Calpastatin.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide to the amino terminal end of the testis isoform of human calpstatin.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric detection system (BCIP/NBT substrates); 1/5000 when using chemoluminescent substrates. Predicted molecular weight: 76 kDa. But note that when used against the reduced protein identifies bands at 70 Kd, 52 Kd, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Sequence similarities
Belongs to the protease inhibitor I27 (calpastatin) family.
Domain
Has four inhibitory domains.
Post-translational
modifications
The N-terminus is blocked.
Target information above from: UniProt accessionP20810
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BS 17 antibody
- Calpain inhibitor antibody
- Calpain inhibitor antibody
see all
Database links
- Entrez Gene: 831 Human
- Entrez Gene: 12380 Mouse
- Entrez Gene: 25403 Rat
- Omim: 114090 Human
- SwissProt: P20810 Human
- SwissProt: P51125 Mouse
- SwissProt: P27321 Rat
- Unigene: 436186 Human
see all
Anti-Calpastatin antibody - Aminoterminal end (Domain L) Testis Isoform images:
Immunocytochemistry/ Immunofluorescence-Calpastatin antibody - Aminoterminal end (Domain L) Testis Isoform(ab28252)
ICC/IF image of ab28252 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28252, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Calpastatin antibody - Aminoterminal end (Domain L) Testis Isoform (ab28252)
All lanes : Anti-Calpastatin antibody - Aminoterminal end (Domain L) Testis Isoform (ab28252) at 1/5000 dilution
Lane 1 : Mouse skeletal tissue muscle lysate
Lane 2 : Mouse skeletal tissue muscle lysate
Lane 3 : Mouse skeletal tissue muscle lysate
Lysates/proteins at 30 µg per lane.
Secondary
HRP-conjugated Goat IgG to Rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 76 kDa
Observed band size : 25,35,74 kDa (why is the actual band size different from the predicted?)
Lanes 1-3 are skeletal muscle samples from different mice.
This image is courtesy of an anonymous Abreview.
Protocols
References for Anti-Calpastatin antibody - Aminoterminal end (Domain L) Testis Isoform (ab28252)
ab28252 has not yet been referenced specifically in any publications.
Publishing research using ab28252? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"