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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Calpastatin antibody - Domain 4A of the long isoform
See all Calpastatin products (11) ...
Description
Rabbit polyclonal to Calpastatin - Domain 4A of the long isoform
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide to domain 4A in the long isoform of human Calpastatin. (Peptide available as ab41391.)
Epitope
ab28255 recognises domains 4A of the long isoform of Calpastatin.
General notes
ab28255 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 76 kDa. ab28255 identifies bands at 110 kDa, 82 kDa and a series of further cleaved active forms when used against the reduced protein. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Sequence similarities
Belongs to the protease inhibitor I27 (calpastatin) family.
Domain
Has four inhibitory domains.
Post-translational
modifications
The N-terminus is blocked.
Target information above from: UniProt accessionP20810
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BS 17 antibody
- Calpain inhibitor antibody
- Calpastatin antibody
see all
Database links
- Entrez Gene: 831 Human
- Omim: 114090 Human
- SwissProt: P20810 Human
- Unigene: 436186 Human
Protocols
References for Anti-Calpastatin antibody - Domain 4A of the long isoform (ab28255)
ab28255 has not yet been referenced specifically in any publications.
Publishing research using ab28255? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"