Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Anti-Calpastatin antibody - Linker between domains 2C and 3A of the long isoform (ab28254)
Overview
Product name
Anti-Calpastatin antibody - Linker between domains 2C and 3A of the long isoform
See all Calpastatin products (11) ...
Description
Rabbit polyclonal to Calpastatin - Linker between domains 2C and 3A of the long isoform
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide to the linker between domains 2C and 3A in the long isoform of human Calpastatin.
(Peptide available as ab41382.)
Epitope
ab28254 recognises the linker between domains 2C and 3A in the Calpastatin long isoform.
Positive control
IL1 beta treated human colorectal adenosarcoma cells lysate.
General notes
ab28254 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
-
Western blot - Calpastatin antibody (ab28254)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 82, 110 kDa (predicted molecular weight: 76 kDa). Higher concentrations of antibody may be needed for samples from more distantly related species Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Sequence similarities
Belongs to the protease inhibitor I27 (calpastatin) family.
Domain
Has four inhibitory domains.
Post-translational
modifications
The N-terminus is blocked.
Target information above from: UniProt accessionP20810
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BS 17 antibody
- Calpain inhibitor antibody
- Calpastatin antibody
see all
Database links
- Entrez Gene: 831 Human
- Entrez Gene: 12380 Mouse
- Entrez Gene: 25403 Rat
- Omim: 114090 Human
- SwissProt: P20810 Human
- SwissProt: P51125 Mouse
- SwissProt: P27321 Rat
- Unigene: 436186 Human
see all
Anti-Calpastatin antibody - Linker between domains 2C and 3A of the long isoform images:
Western blot - Calpastatin antibody (ab28254)
Anti-Calpastatin antibody - Linker between domains 2C and 3A of the long isoform (ab28254) at 1/1000 dilution + IL1 beta treated human colorectal adenosarcoma cell lysate.
Predicted band size : 76 kDa
Observed band size : 82,110 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Calpastatin antibody - Linker between domains 2C and 3A of the long isoform (ab28254)
This product has been referenced in:
- Selsby Jet al. Leupeptin-based inhibitors do not improve the mdx phenotype. Am J Physiol Regul Integr Comp Physiol 299:R1192-201 (2010). WB; Mouse.Read more (PubMed: 20844259) »
See 1 publication for this product
Publishing research using ab28254? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"