Anti-Calpastatin antibody (ab28252)
Key features and details
- Rabbit polyclonal to Calpastatin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Calpastatin antibody
See all Calpastatin primary antibodies -
Description
Rabbit polyclonal to Calpastatin -
Host species
Rabbit -
Specificity
ab28252 recognises Calpastatin aminoterminal end (Domain L) testis isoform -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide to the amino terminal end of the testis isoform of human calpstatin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) | |
ICC/IF |
Notes |
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WB: 1/1000 when using colorimetric detection system (BCIP/NBT substrates); 1/5000 when using chemoluminescent substrates. Predicted molecular weight: 76 kDa. But note that when used against the reduced protein identifies bands at 70 Kd, 52 Kd, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. -
Sequence similarities
Belongs to the protease inhibitor I27 (calpastatin) family. -
Domain
Has four inhibitory domains. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
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Database links
- Entrez Gene: 831 Human
- Entrez Gene: 12380 Mouse
- Entrez Gene: 25403 Rat
- Omim: 114090 Human
- SwissProt: P20810 Human
- SwissProt: P51125 Mouse
- SwissProt: P27321 Rat
- Unigene: 436186 Human
see all -
Alternative names
- BS 17 antibody
- Calpain inhibitor antibody
- Calpastatin antibody
see all
Images
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ICC/IF image of ab28252 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28252, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab28252 has been referenced in 8 publications.
- Zeng X et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
- Popova S et al. Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal. Sci Rep 10:15185 (2020). PubMed: 32938992
- de la Fuente S et al. Calpain system is altered in survival motor neuron-reduced cells from in vitro and in vivo spinal muscular atrophy models. Cell Death Dis 11:487 (2020). PubMed: 32587237
- Pánico P et al. Arsenic impairs GLUT1 trafficking through the inhibition of the calpain system in lymphocytes. Toxicol Appl Pharmacol 380:114700 (2019). PubMed: 31398423
- Lin XY & Chen SZ Calpain inhibitors ameliorate muscle wasting in a cachectic mouse model bearing CT26 colorectal adenocarcinoma. Oncol Rep 37:1601-1610 (2017). PubMed: 28112357
- Zeng X et al. Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells. Oncotarget 8:72872-72885 (2017). PubMed: 29069832
- Kemp CM et al. The effects of Capn1 gene inactivation on skeletal muscle growth, development, and atrophy, and the compensatory role of other proteolytic systems. J Anim Sci 91:3155-67 (2013). PubMed: 23798514
- Cunha TF et al. Aerobic exercise training upregulates skeletal muscle calpain and ubiquitin-proteasome systems in healthy mice. J Appl Physiol 112:1839-46 (2012). PubMed: 22461440