Overview

  • Product nameAnti-Calponin antibody [CALP]See all Calponin primary antibodies ...
  • Description
    Mouse monoclonal [CALP] to Calponin
  • Tested applicationsIHC-Fr, Flow Cyt, ICC/IF, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Crude human uterus extract

  • Positive control
    • Myoepithelium in normal breast glands.

Properties

Applications

Our Abpromise guarantee covers the use of ab700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/400. ABC system. 30mins at RT.
Calponin has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin has also been useful in malignant myoepithelium and pleomorphoric adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions.
Flow Cyt Use at an assay dependent dilution. PubMed: 20618694
ICC/IF Use at an assay dependent dilution.
IHC-FoFr Use at an assay dependent dilution. PubMed: 20138661
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC system. 30mins at RT.

Target

  • FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Tissue specificitySmooth muscle, and tissues containing significant amounts of smooth muscle.
  • Sequence similaritiesBelongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic calponin antibody
    • Calponin 1 antibody
    • Calponin 1 basic smooth muscle antibody
    • Calponin H1 antibody
    • Calponin H1 smooth muscle antibody
    • Calponin-1 antibody
    • Calponin1 antibody
    • Calponins basic antibody
    • CNN 1 antibody
    • Cnn1 antibody
    • CNN1_HUMAN antibody
    • Sm Calp antibody
    • SMCC antibody
    • smooth muscle antibody
    see all

Anti-Calponin antibody [CALP] images

  • ab700 at a 1/50 dilution staining rat heart tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed prior to incubation with the antibody overnight at 4°C. An Alex-Fluor ® 488 conjugated  goat anti-mouse antibody was used as the secondary. The image was taken  with a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode and shows stained Calponin (smooth muscle cells-green) and counterstained nuclei (DAPI-blue) (left panel).DIC (phase) image of the same tissue (right panel) confirm the localization of the calponin staining in the arterial smooth muscle in the heart tissue with very little background.

    See Abreview

  • ab700 staining Calponin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Alexa Fluor®488-conjugated chicken polyclonal to mouse IgG was used as secondary antibody at 1/400 dilution. Image shows combined fluorescent and transmitted light images. Hoechst 33342 was used to stain the cell nuclei (blue).

    See Abreview

References for Anti-Calponin antibody [CALP] (ab700)

This product has been referenced in:
  • Wu W  et al. Artificial niche combining elastomeric substrate and platelets guides vascular differentiation of bone marrow mononuclear cells. Tissue Eng Part A 17:1979-92 (2011). WB, IHC-Fr ; Rat . Read more (PubMed: 21449713) »
  • Hua X  et al. Expression and role of fibroblast activation protein-alpha in microinvasive breast carcinoma. Diagn Pathol 6:111 (2011). IHC-P ; Human . Read more (PubMed: 22067528) »

See all 9 Publications for this product

Product Wall

The first reference on the datasheet contains the only western blot I have found for this clone, in Figure 8, but it is cropped.

Wu W et al. Artificial niche combining elastomeric substrate and platelets guides vascular differentiation of bo...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Smooth muscle cells from mesenteric artery)
Specification Smooth muscle cells from mesenteric artery
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 17 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart tissue)
Specification Heart tissue
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Mal Niladri

Verified customer

Submitted Oct 16 2006

As far as we are aware, this antibody detects all three.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"