Overview

  • Product nameAnti-Calponin antibody [EP798Y]
    See all Calponin primary antibodies
  • Description
    Rabbit monoclonal [EP798Y] to Calponin
  • Tested applicationsWB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to residues near the C-term of Calponin (Human).

  • Positive control
    • Human bladder lysate; HeLa cells; Human smooth muscle tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab46794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 34 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration.
  • Application notesIs unsuitable for IP.
  • Target

    Anti-Calponin antibody [EP798Y] images

    • Anti-Calponin antibody [EP798Y] (ab46794) at 1/20000 dilution + Human bladder lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of Hela cells using ab46794 at 1/100 dilution
    • Immunohistochemical staining of paraffin-embedded human smooth muscle using ab46794 at 1/100 dilution
    • ab46794 staining Calponin in porcine aortic smooth muscle cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100. Samples were then incubated with primary antibody at 1/50 for 1 hour at 25°C. The secondary antibody used was ab6717 Goat polyclonal to Rabbit IgG - H&L (FITC) (green) used at a 1/400 dilution.

      See Abreview

    • ICC/IF image of ab46794 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46794, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-Calponin antibody [EP798Y] (ab46794) at 1/20000 dilution

      Lane 1 : Whole tissue lysate prepared from bovine lymph vessels
      Lane 2 : Whole tissue lysate prepared from bovine lymph vessels

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
      developed using the ECL technique

      Observed band size : 34 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Image courtesy of an anonymous Abreview.

      See Abreview

    • ab46794 showing positive staining in Normal kidney vessels tissue.

    • ab46794 showing positive staining in Normal lung vessel tissue.

    • ab46794 showing positive staining in Normal tonsil vessel tissue.

    • ab46794 showing positive staining in Normal uterus tissue.

    • ab46794 showing negative staining in Skeletal muscle tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Calponin antibody [EP798Y] (ab46794)

    This product has been referenced in:
    • Battiston KG  et al. Monocyte/macrophage cytokine activity regulates vascular smooth muscle cell function within a degradable polyurethane scaffold. Acta Biomater 10:1146-55 (2014). Read more (PubMed: 24361424) »
    • Nyp MF  et al. TRIP-1 via AKT modulation drives lung fibroblast/myofibroblast trans-differentiation. Respir Res 15:19 (2014). WB ; Human . Read more (PubMed: 24528651) »

    See all 29 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (vascular smooth muscle cell)
    Specification vascular smooth muscle cell
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

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    Verified customer

    Submitted Jul 07 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (11% SDS-PAGE)
    Sample Human Cell lysate - whole cell (HUVECells and 5ng/ml TNF-a for 24hr)
    Specification HUVECells and 5ng/ml TNF-a for 24hr
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Aug 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (smooth muscle cells)
    Specification smooth muscle cells
    Fixative Acetone
    Permeabilization Yes - acetone permeabilizes as well
    Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 25% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 18 2012

    Thank you for contacting us.

    In order to redeem the free antibody, please use the code that was provided to you (xxx) on your next order that you place with Abcam (before the expiration date: xxx).

    As per instructions:
    The code wi...

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    Thank you foryour phone call.

    Below is the information about the testing discount program that I had mentioned as well as your discount code.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you woul...

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    Thank you for contacting us.

    You would air-dry your section after placing them on the slide, and then store them at -20 or -80 degree in a airtight box to prevent water condensation during storage.


    I hope this information is ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (primary myometrial cells)
    Specification primary myometrial cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X in PBS
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

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    Verified customer

    Submitted Aug 07 2012

    Thank you for contacting us.

    The MSDS for ab46794 is attached.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. ...

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    Thank you for contacting us.

    ab683 does not contain any preservative so MSDS is not required for this.

    ab14106 MSDS can be downloaded online for m the datasheet. Please also find attached;

    ab46794. I will send you the MSDS so...

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    Thank you for contacting Abcam.

    Please find links below to a few antibodies that we have that recognize smooth muscle heavy chain 1 and 2:

    http://www.abcam.com/Myosin-smooth-muscle-heavy-chain-1-and-2-antibody-EPR5335-ab124679.html ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"