Anti-Calreticulin antibody (ab94935)
Key features and details
- Chicken polyclonal to Calreticulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgY
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Overview
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Product name
Anti-Calreticulin antibody
See all Calreticulin primary antibodies -
Description
Chicken polyclonal to Calreticulin -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Pig, Zebrafish, Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Mouse Calreticulin aa 24-43.
Sequence:KEQFLDGDAWTNRWVESKHK
(Peptide available asab5838) -
Positive control
- WB: A-549, HEK-293 cell lysate; Mouse Brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
Sequence similarities
Belongs to the calreticulin family. -
Domain
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localization
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
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Database links
- Entrez Gene: 281036 Cow
- Entrez Gene: 102121336 Cynomolgus monkey
- Entrez Gene: 811 Human
- Entrez Gene: 12317 Mouse
- Entrez Gene: 100381266 Pig
- Entrez Gene: 100009050 Rabbit
- Entrez Gene: 30248 Zebrafish
- Omim: 109091 Human
see all -
Alternative names
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
Images
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All lanes : Anti-Calreticulin antibody (ab94935) at 1/2000 dilution
Lane 1 : A-549 cell lysate
Lane 2 : HEK-293 cell lysate
Lane 3 : Mouse Brain lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml
Predicted band size: 48 kDa
Datasheets and documents
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Datasheet download
References (1)
ab94935 has been referenced in 1 publication.
- Stamm CE et al. Screening Mycobacterium tuberculosis Secreted Proteins Identifies Mpt64 as a Eukaryotic Membrane-Binding Bacterial Effector. mSphere 4:N/A (2019). PubMed: 31167949