Calretinin antibody [SP13] (ab16694)

Product Name 

Calretinin antibody [SP13]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [SP13] to Calretinin

Immunogen 

Recombinant full length calretinin protein (Mouse).

Reacts with 

Hu, Rat

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Specificity 

Ab16694 recognises Calretinin.

Tested applications 
(see key)

ICC/IF, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/200 (PMID 19701198).
IHC-P: 1/100 for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human mesothelioma for Immunohistochemistry.

Cellular localization 

Cytoplasmic

Research areas 

Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins

Relevance 

Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. The antibodies against calretinin also labels the mossy fibers throughout, and climbing fibers in a distinct group of folia. They also stain several cell types in the molecular layer. There is no appreciable immunostaining of calretinin in cerebellar cortex. In the rat, antibodies against calretinin stain the Lugaro cells, and some granule cells in lobe X; they also give weak staining of all the granule cells in the other lobes. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

SP13

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide.
Constituents: Tissue culture supernatant.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

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Calretinin antibody [SP13] images:

References for Calretinin antibody [SP13] (ab16694)

  This product has been used in:

Kuwabara T et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci 12:1097-105 (2009). ICC/IF; Rat. PubMed: 19701198

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