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Cancer Antigen CA125 Human ELISA Kit (ab108653)

Submit an Abreview Q&A (8)

Overview

  • Product nameCancer Antigen CA125 Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeSandwich
  • Sensitivity
    = 5 U/ml
  • Range
    5 U/ml - 400 U/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab108653 is for the quantitative determination of the Cancer Antigen CA125 concentration in human serum.

    Cancer Antigen 125 (CA125) is a surface antigen associated with epithelial ovarian cancer. In serum, CA125 is associated with a high molecular weight glycoprotein. Published studies have indicated that elevated serum CA125 levels can be found in individuals with serious endometroid, clear-cell and undifferentiated ovarian carcinoma.

    The serum CA125 concentration is greater than 35 units per ml in 60% of women with ovarian cancer and >80% of patients with disseminated ovarian cancer. The serum CA125 is elevated in 1% of normal healthy women, 3% of normal healthy women with benign ovarian diseases, and 6% of patients with non-neoplastic conditions (including but not limited to first trimester pregnancy, menstruation, endometriosis, uterine fibrosis, acute salphingitis, hepatic diseases and inflammation of peritoneum, pericardium or pleura). Serum CA125 concentration may be useful in monitoring treatment and distinguishing between good response to treatment and progressive malignant disease with poor therapeutic response. To date, CA125 is the most sensitive marker for residual epithelial ovarian cancer.

  • Tested applicationsELISA more details

Properties

  • FunctionThought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • Tissue specificityExpressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • Sequence similaritiesContains 2 ANK repeats.
    Contains 56 SEA domains.
  • DomainComposed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • Post-translational
    modifications    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • Cellular localizationCell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

    • Target information above from: UniProt accession Q8WXI7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CA 125CA-125CA125
        MUC 16MUC-16MUC16MUC16_HUMANMucin 16Mucin-16Ovarian cancer related tumor marker CA125Ovarian cancer-related tumor marker CA125Ovarian carcinoma antigen CA125
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108653 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Cancer Antigen CA125 Human ELISA Kit images

      • ELISA - Cancer Antigen CA125 Human ELISA Kit (ab108653)
        ELISA - Cancer Antigen CA125 Human ELISA Kit (ab108653)
        Example Cancer Antigen CA125 standard curve

      Protocols

      References for Cancer Antigen CA125 Human ELISA Kit (ab108653)

      ab108653 has not yet been referenced specifically in any publications.

      Publishing research using ab108653 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108653 Submit a Question for ab108653

      Displaying 1 - 8 of 8 results for Abreviews and Q&A

      10
      Votes: Highest First

      Are dilution linearity studies available for this product?
      They wish to test in a yet untested species and will be diluting the samples. What diluent would we recommend?

      Attached is the dilution linearity data for this antibody. The sample can be diluted with the zero standard.

      Hola :
      El kit CA-125 con catálogo ab108653 podría ser usado en plasma? Es
      recomendado para suero, pero el cliente desea usarlo en plasma y quiere
      saber si se afectaría el resultado o si le serviría?
      Gracias....

      Read More

      Gracias por contactarnos de nuevo.

      Me confirman mis compañeros del laboratorio que este kit en principio solo se ha validado con muestras de suero. Es muy probable que sea válido para usar con muestras de plasma, pero al no haberlo...

      Read More

      What is the clone number of the monoclonal antibody in the kit?

      Thank you for contacting us.

      I received the following information from the supplying lab:

      The two monoclonal antibodies used in the ab108653 ELISA kit are specific for the antigenic B-domain, similar to M-11.

      I hope this informa...

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      Are the antibodiesavailable for sale separately?

      Thank you for your call yesterday and for your patience while I have been in touch with the lab.

      Unfortunately the antibodies used in this kit are not available for sale separately.

      I am sorry that I can't be more help in this situatio...

      Read More

      Sorry for the delay in responding to you. I've been dealing with some other stuff.

      Thank you very much for your patience.
      After having discussion with lab and my other colleagues regrettably I have to say we can't provide the product, separate than the kit. I apologize for any inconvenience caused.
      I would suggest searching th...

      Read More

      Sorry for the delay in responding to you. I've been dealing with some other stuff.

      We are considering your request as high priority. We will get back to you with all the information soon.
      Many thanks for having patience!

      Hello,
      I have a question about your CA125 ELISA Kit.
      We are interested in doing some initial testing with
      standardized materials. To that end, I'd like to know whether it would
      be possible to purchase some of your CA125 Reference Stand...

      Read More

      Thank you for your enquiry and your interest in our products.
      We prove the CA125 reference standards for this kit in different concentrations 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 6 x 1ml. Unfortunately, currently we are unable to sell the co...

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       Dear technical team,   Our customer would like to purchase these kits and wants to know the meaning of "unit"  in Sensitivity : 5U/ml.   1. unit definition   2. is it possible conversion of unit  to  ng?   Check this please.     Best regards,

      Thank you for your enquiry. I can confirm that this kit is sourced externally. They have kindly provided the following information: 1. When this kit was first designed, the antigens used were not pure.  So the antigen concentrations was expressed in U/ml....

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"