Candida albicans IgG Human ELISA Kit (ab108715)
- Product nameCandida albicans IgG Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeIndirect
- Species reactivityReacts with: Human
- Product overview
ab108715, Candida albicans IgG Human ELISA Kit is intended for the quantitative determination of IgG-class antibodies against Candida albicans in Human serum or plasma.
Candida albicans is a ubiquitious yeast-like fungus belonging to the family of ascomycota. It is a facultative pathogen belonging to the normal microbial flora of skin and mucosa. Beside the yeast form which appears mainly with superficial infections, so called pseudomycelia are another morphological form of ascomycota. Pseudomycelia prevail with invasive mycosis. Although not pathogenic in healthy Humans the fungus may be opportunistic in those suffering from a variety of disorders, and in those treated intensively with broad-spectrum antibiotics or immunosuppressive measures. Candidiasis is caused to about 90% by C. albicans. It is an acute, superficial or subacute, invasive infection. Acute infections with the fungus may produce lesions in the mouth, vagina, skin and nails. Subacute infections may affect bronchi, lungs, heart or meningeal. In immunosuppressant patients with cellular immunodeficiency, e.g., AIDS patients, C. albicans may lead to severe necroses of infected tissues.
Symptoms: Depending upon the location of C. albicans infectious the kind of lesion varies. Oral infections for example show typical creamy white patches of trush whereas in bronchial candidiasis cough is the most characteristic symptom.
Mechanism of Infection:-
Endogene: Usually candidiasis is due to increased susceptibility to a member of the normal Human flora.
Exogene: Transmission between individuals: Infection of newborns from the birth canal; transmission by nonsterile hospital equipment.
ab108715 is based on ELISA (Enzymelinked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with Candida albicans antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added. This conjugate binds to the captured Candida albicans-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Candida albicans -specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
- Tested applicationsIndirect ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests Candida albicans anti-IgG Conjugate Blue with Black cap 1 x 20ml Candida albicans Coated Wells (IgG) Plate 1 unit Candida albicans IgG Cut-off Control Yellow with Green cap 1 x 3ml Candida albicans IgG Negative Control Yellow with Blue cap 1 x 2ml Candida albicans IgG Positive Control Yellow with Red cap 1 x 2ml IgG Sample Diluent Yellow with white cap 1 x 100ml Stop Solution Red Cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml Washing Solution 20X White cap 1 x 50ml
- Research Areas
- C albicansC. albicansCandida albicansCandida albicans IgG
Our Abpromise guarantee covers the use of ab108715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Candida albicans IgG Human ELISA Kit (ab108715)
ab108715 has not yet been referenced specifically in any publications.