Anti-Candida albicans IgG Human ELISA Kit (ab108715)

Overview

  • Product nameAnti-Candida albicans IgG Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Candida albicans IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Candida albicans in Human serum and plasma.

     

    A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.

  • Tested applicationsIndirect ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • C albicans
    • C. albicans
    • Candida albicans
    • Candida albicans IgG

Applications

Our Abpromise guarantee covers the use of ab108715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References for Anti-Candida albicans IgG Human ELISA Kit (ab108715)

ab108715 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Plasma samples were all diluted 1:100 as suggested in the manual. All other steps were performed according to the instructions.
This ELISA is very fast (pre-coated wells and no blocking step required), however one has to keep in mind that it does not provide quantitative data. The optical densities of the samples are proportionate to the anti-C.albicans IgG levels in the samples. A positive control, a negative control and a cut-off control are provided in order to check the ELISA is working.
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Submitted Jul 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"