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Anti-Candida albicans antibody (FITC) (ab21164)

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Overview

  • Product nameAnti-Candida albicans antibody (FITC)See all Candida albicans primary antibodies ...
  • Description
    Rabbit polyclonal to Candida albicans (FITC)
  • ConjugationFITC
  • SpecificityRecognizes numerous proteins in a soluble C. albicans extract (IEP). Has not been absorbed and does cross-react with other yeasts. Negative against human serum, urine and spinal fluid.
  • Tested applicationsDouble Immunodiffusion, Counter Current Immunoelectrophoresis, ICC/IF, ELISA more details
  • Species reactivity
    Has not been absorbed and does react with other yeasts.
  • Immunogen

    Candida albicans, type A (ATCC #32354).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesProtein A chromatography purified IgG fraction covalently coupled with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab21164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Double Immunodiffusion
Counter Current Immunoelectrophoresis
ICC/IF
ELISA
  • Application notesCCIe: Use at an assay dependent dilution.
    DID: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.

    Use neat in gel-precipitin reactions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceCandida albicans is the most frequently isolated fungal pathogen of humans, affecting immunocompromised patients ranging from premature infants to AIDS sufferers. Systemic infections have an attributed mortality of 30-50%. C. albicans is a diploid organism which has eight sets of homologous chromosomes. It has a genome of approximately 16 Mb (haploid), about 30% greater than S. cerevisiae (baker’s yeast).
    • Alternative names
        C albicans antibodyC. albicans antibodyThrush antibody

    References for Anti-Candida albicans antibody (FITC) (ab21164)

    ab21164 has not yet been referenced specifically in any publications.

    Publishing research using ab21164 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab21164 Submit a Question for ab21164

    Displaying 1 - 8 of 8 results for Abreviews and Q&A

    10
    Votes: Highest First

    Is there a protocol for staining cultured C. albicans yeast?

    Per the lab ab20005 does stain C. albicans cells, but usually the FITC version (ab21164) is used. The biotin version should still work well when used with Streptavidin-FITC instead of a secondary FITC-labelled antibody, and using biotin-streptavidin shoul...

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    I tested the antibody and it works for FACS (please see attached PDF) The blue histogram corresponds to anti-Candida biotin Ab staining followed by streptavidin-APC staining (each 30 min at 4oC). The histograms with black (Candida alone without any stai...

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    Thank you for contacting us. I am very glad to hear that this product worked so well in FACS.

    After submitting the Abreview your code will become 'active'. When you place your next order use the normal system costs and input this code. The code...

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    I just placed an order for the ab53892 Ab. Please let me know where in your website can I access the Abreview section to report on whether it works for FC or not

    I am glad that you have decided to take advantage of our 100% Abreview program. To place an Abreview you will need to go to the webpage for the product and click on the 'ABREVIEW' tab just below the shipping information. This tab will contain a link to &q...

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    Thank you Keith It sounds that the only antibody with exclusively extracellular staining is the ab53892 correct? John has emailed me yesterday that the other two Abs (ab20005, ab21164) are both intra- and extracellular. If the above is correct (please...

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    Thank you for contacting us. Ab53892 is the only product of these three which is against the extracelluar region specifically. I have created an Abreview discount code for you should you desire to use it. There is not obligation to do so. I hope thi...

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    Ok, please provide me feedback for the item ab53892 as well and then i ll decide on which of the three I will proceed purchasing To clarify, i would buy this anti-Candida antibody for the full amount but then I can order any primary antibody from Abcam f...

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    Thank you very much for your patience as I contacted the lab. The staining pattern forab53892 is extracellular. As to our testing program, you are correct. You will receive a code good for one free antibody of your choice which will become active once ...

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    Thank you for getting back to me I would be interested in testing the antibodies you mention as long as the staining pattern on the intact cells was surface. If you could send me a sample small aliquot or if I could get them for a low price to test them...

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    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    Therefore, I can offer a discount off a future purchase if you buyab20005 and/or ab21164 and test in FCand ...

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    Want to do flow cytometry and would like to know if these antibodies recognises an intra- or extracellular epitope.

    Thank you for contacting Abcam and for your interest in our Anti-Candida albicans antibodies.


    Catalog #s ab2005 and ab21164 are polyclonal antibodies and therefore will recognize multiple epitopes on multiple proteins. The immunogen was a ...

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    Thank you for your reply. I have a few questions. 1.The polyclonal antibodies that you offer are all labeled, what about unlabeled polyclonal antibodies? 2. What does it mean that the antibody reacts with other yeasts? Does it mean that will react wit...

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    Thank you for your email. The only non-conjugated Candida albicans antibody that we have is ab23368 (Mouse monoclonal [6401] to Candida albicans). We don't have an unconjugated polyclonal antibody. For ab20005, ab20028 and ab21164, the antibodies will c...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"