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|Sample type||Average %||Range|
|Serum||> 85||15.6pg/ml - 1000pg/ml|
NGAL (ab205085) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of NGAL protein in biological samples of dogs.
In this assay the NGAL present in samples reacts with the anti-NGAL antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-NGAL, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of NGAL in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of NGAL in the test sample. The quantity of NGAL in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|100X Detection Antibody Conjugate||1 x 150µl|
|100X HRP Streptavidin||1 x 150µl|
|1X Diluent Concentrate||1 x 60ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Anti-Dog NGAL ELISA Microplate||1 unit|
|Chromogen Substrate Solution||1 x 12ml|
|DOG NGAL Calibrator (Lyophilized)||1 vial|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab205085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab205085 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"