Overview

  • Product name
    Anti-Cannabinoid Receptor I antibody
    See all Cannabinoid Receptor I primary antibodies
  • Description
    Goat polyclonal to Cannabinoid Receptor I
  • Specificity
    This antibody is expected to recognise both reported isoforms (NP_057167.2 and NP_149421.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide:

    HTSEDGKVQVTRPDQ

    , corresponding to Internal sequence amino acids 320-334 of Human Cannabinoid Receptor I

  • Positive control
    • Rat brain lysate. Lysate prepared from transfected HEK293 cells over-expressing Cannabinoid Receptor I.

Properties

Applications

Our Abpromise guarantee covers the use of ab40860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa). 65kDa band detected in rat brain lysate.
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343).

Target

Images

  • Anti-Cannabinoid Receptor I antibody (ab40860) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).

    Predicted band size : 53 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:
  • Kim J  et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol 5:100 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24711795) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (synovial fibroblasts from osteoarthritic person)
Specification
synovial fibroblasts from osteoarthritic person
Preparation
Cell harvesting/tissue preparation method: cells were detached by accutase (PAA), spun and fixed in 4 % formalin for 20 min. Then, cells were spun and resuspended in 100 µl BD Perm/Wash solution containing 0,5 µg CB1 (ab40860). This was then incubated for 30 min at 4 degrees. Samples were washed with 1ml of BD Perm/Wash buffer and spun again. Then 100 µl of BD Perm/wash were added including 1 µl secondary antibody (donkey anti goat, Santa Cruz Biotech). This was again incubated at 4 degrees for 30 min. After another wash step, cells were resuspended in 4 % Formalin in PBS and analyzed.
Sample buffer: BD Perm/Wash, 4 % Formaldehyde in PBS
Fixation
Formaldehyde
Permeabilization
Yes - BD Perm/Wash
Gating Strategy
fibroblast culture only
Username

Dr. Torsten Lowin

Verified customer

Submitted Apr 16 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Recombinant protein (CHO cells expressing human CB1 receptor)
Loading amount
60000 cells
Specification
CHO cells expressing human CB1 receptor
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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