Anti-Cannabinoid Receptor II antibody (ab3561)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cannabinoid Receptor II antibody (ab3561)

ab3561 at 1/5000 staining rat spinal cord tissue sections by IHC-P. Following nerve trans-section, the animal was transcardially perfused with PBS followed by 4% formaldehyde (no post fixation) then frozen in OCT and stored at -80°C until required. 20µm sections were cut and stained with the antibody for 18 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy

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  Immunocytochemistry/ Immunofluorescence - Cannabinoid Receptor II antibody (ab3561)

ab3561 staining Cannabinoid Receptor II in murine BV2 cells by Immunocytochemistry/ Immunofluorescence. Cells were stimulated with 10 uM ADP for 15 minutes at 37°C, 5%CO₂ then washed 3X with PBS and fixed with 2% formaldehyde for 15 minutes at room temperature. A blocking step was performed for 1 hour at room temperature with 5% FBS/PBS/0.01% Triton-X-100. ab3561 used at a 1/500 dilution for 1 hour at room temperature then washed 3X PBS. The secondary was an Alexa Fluor 488 conjugated antibody, used at a 1/250 for 1 hour at room temperature, washed 3X PBS then mounted in Vectashield containing DAPI.

Image courtesy of an anonymous Abreview.

  Flow Cytometry - Anti-Cannabinoid Receptor II antibody (ab3561)

Quantification of Cannabinoid Receptor II surface expression in primary human and HaCaT keratinocytes. FL-1 shows autofluorescence (black population), non-specific binding of secondary antibody (dark grey population) and specific immunofluorescence obtained with ab3561, respectively (light grey population).

Image from Leonti M et al, Biochem Pharmacol. 2010 Jun 15;79(12):1815-26. Epub 2010 Mar 3, Fig 6. DOI 10.1016/j.bcp.2010.02.015