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I need a anti-rat biotin conjugated secondary Ab raised in goat to perform IHC on human joint tissue. In addition to ab6275, you have ab6619, 6256, 7176 in your catalog that match that description. Can you tell me if there is any difference between these antibodies and which one you recommend for this application (IHC)?
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ANSWER: |
Thank you for your enquiry. Ab6275: Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(ab')2 [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(ab')2. Ab7176: Biotin Conjugated Fab Fragment of Affinity Purified anti-Rat IgG [H&L] [Goat] - This product is the Fab portion (single Fab fragments) of the Goat IgG raised against Rat IgG. Ab6256:Biotin Conjugated F(ab')2 Fragment of Affinity Purified anti-Rat IgG F(c) [Goat] Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins - This product is the F(ab')2 portion of the goat IgG that is raised against Rat F(c). Typically Fab and Fab2 fragments will bind less than the entire IgG molecule. This means these types of products are good for assays in which very low background is desired. I hope I was able to make things more clear. Please do not hesitate to contact me if you have any other questions regarding this issue. |
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Customer received vial and there was only 65 ul in it (should have been 100 ul). Order# 32618. |
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ANSWER: |
I apologize that you were shorted in your vial of ab6619. I am sending you another vial free of charge and you should receive it tomorrow. For your record it's order 33266. Let me know if there are any more problems. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa (possible non-specific binding).
ab6619 at 1/2000 staining Carbonic Anhydrase I by Western Blot.
Lanes 1-3 are purified CA-I. The other lanes are 1ul human vitreous (1-3 ug protein). NDM: no diabetic patient, noDR: diabetic patient no diabetic retinopathy, QPDR: quiescent proliferative diabetic retinopathy, PDR: proliferative diabetic retinopathy. A HRP conjugated donkey anti-goat antibody was used as the secondary. The western blot shows that CA-I was only detected in vitreous from PDR and QPDR patients. (See Gao, et al. NATURE MEDICINE, 2007;13(2);181-188). Because the samples differ between patients and the severity of hemorrhage are also different so the intensity of the bands varies a lot. Another band was detected around 55 kDa and this was confirmed to be IgG.
This image is courtesy of an anonymous Abreview
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
ab6619 at 1/2000 staining Carbonic Anhydrase I by Western Blot.
Lanes 1-4 shows Carbonic Anhydrase I (positive control). Rat RBCs and human RBCs were loaded at 0.01ul. An HRP conjugated anti-goat antibody was used as the secondary.
Only 1 band at around 30kDa was detected using RBCs.
This image is courtesy of an anonymous Abreview
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