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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-Carbonic Anhydrase I antibody
See all Carbonic Anhydrase I products (16) ...
Description
Goat polyclonal to Carbonic Anhydrase I
Specificity
No cross-reactivity to carbonic anhydrase II.
Tested applications
IP, ELISA, Conjugation, Dot Blot, WBmore details
Cross reactivity
Reacts with
Rabbit, Human
Predicted to work with
a wide range of other species
Immunogen
Carbonic Anhydrase I [Human Erythrocytes]
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
ELISA
Conjugation
Dot Blot
Application notes
immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity
Target
Function
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00915
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- JW0122 antibody
- CA 1 antibody
- CA I antibody
see all
Database links
- Entrez Gene: 759 Human
- Omim: 114800 Human
- SwissProt: P00915 Human
- SwissProt: P07452 Rabbit
- Unigene: 23118 Human
- Unigene: 1836 Rabbit
Anti-Carbonic Anhydrase I antibody images:
Western blot - Carbonic Anhydrase I antibody (ab6619)
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa (possible non-specific binding).
ab6619 at 1/2000 staining Carbonic Anhydrase I by Western Blot.
Lanes 1-3 are purified CA-I. The other lanes are 1ul human vitreous (1-3 ug protein). NDM: no diabetic patient, noDR: diabetic patient no diabetic retinopathy, QPDR: quiescent proliferative diabetic retinopathy, PDR: proliferative diabetic retinopathy. A HRP conjugated donkey anti-goat antibody was used as the secondary. The western blot shows that CA-I was only detected in vitreous from PDR and QPDR patients. (See Gao, et al. NATURE MEDICINE, 2007;13(2);181-188). Because the samples differ between patients and the severity of hemorrhage are also different so the intensity of the bands varies a lot. Another band was detected around 55 kDa and this was confirmed to be IgG.
This image is courtesy of an anonymous Abreview
Western blot - Carbonic Anhydrase I antibody (ab6619)
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
ab6619 at 1/2000 staining Carbonic Anhydrase I by Western Blot.
Lanes 1-4 shows Carbonic Anhydrase I (positive control). Rat RBCs and human RBCs were loaded at 0.01ul. An HRP conjugated anti-goat antibody was used as the secondary.
Only 1 band at around 30kDa was detected using RBCs.
This image is courtesy of an anonymous Abreview
References for Anti-Carbonic Anhydrase I antibody (ab6619)
ab6619 has not yet been referenced specifically in any publications.
Publishing research using ab6619? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"