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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-Carbonic Anhydrase II antibody
See all Carbonic Anhydrase II products (9) ...
Description
Sheep polyclonal to Carbonic Anhydrase II
Specificity
This product is specific by double diffusion when tested against CAI and CAII antigens. When tested by Western blot, cross-reactivity with a commercially available CAI has been observed. It is unclear if this is due to the antibody cross-reacting with CAI or if the CAI preparation is contaminated with CAII, both of which are purified from erythrocytes.
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rabbit, Human
Immunogen
Human Carbonic Anhydrase II, purified from human erythrocytes.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Antiserum is prepared by immunisation of sheep with Human Carbonic Anhydrase II and, if necessary, adsorption to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is finally 0.2µm filtered.
Primary antibody notes
Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration.
IHC-Fr: 1/700(PubMed: 16932748)
Target
Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
Involvement in disease
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00918
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CA 2 antibody
- CA II antibody
- CA-II antibody
see all
Database links
- Entrez Gene: 760 Human
- Entrez Gene: 12349 Mouse
- Omim: 259730 Human
- SwissProt: P00918 Human
- SwissProt: P00920 Mouse
- SwissProt: P00919 Rabbit
- Unigene: 155097 Human
- Unigene: 1186 Mouse
References for Anti-Carbonic Anhydrase II antibody (ab8953)
This product has been referenced in:
- Paunescu TGet al. V-ATPase expression in the mouse olfactory epithelium. Am J Physiol Cell Physiol : (2008). IHC-Fr; Mouse.Read more (PubMed: 18667600) »
- Blomqvist SRet al. Epididymal expression of the forkhead transcription factor Foxi1 is required for male fertility. EMBO J 25:4131-41 (2006).Read more (PubMed: 16932748) »
See all 2 publications for this product
Publishing research using ab8953? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"