![Western blot - Carbonic Anhydrase III antibody [2CA-4] (ab49111) image](#Carbonic-Anhydrase-III-Primary-antibodies-ab49111-2.jpg)
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Products:Cardiovascular >> Hypoxia >> Associated Proteins
Overview
Product name
Anti-Carbonic Anhydrase III antibody [2CA-4]
See all Carbonic Anhydrase III products (5) ...
Description
Mouse monoclonal [2CA-4] to Carbonic Anhydrase III
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human)
Positive control
This antibody gave a positive signal in human skeletal muscle tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.3
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
2CA-4
Isotype
IgG1
Light chain type
kappa
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Cardiovascular >> Hypoxia >> Associated Proteins
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Western blot - Carbonic Anhydrase III antibody [2CA-4] (ab49111)
![Western blot - Carbonic Anhydrase III antibody [2CA-4] (ab49111) image](/ps/datasheet/images/49/ab49111/Carbonic-Anhydrase-III-Primary-antibodies-ab49111-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA: Use at an assay dependent dilution.
Target
Function
Reversible hydration of carbon dioxide.
Tissue specificity
Muscle specific.
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Developmental stage
At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
Post-translational
modifications
S-glutathionylated in hepatocytes under oxidative stress.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP07451
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CA III antibody
- CA-III antibody
- Ca3 antibody
see all
Database links
- Entrez Gene: 761 Human
- Omim: 114750 Human
- SwissProt: P07451 Human
- Unigene: 82129 Human
Anti-Carbonic Anhydrase III antibody [2CA-4] images:
Western blot - Carbonic Anhydrase III antibody [2CA-4] (ab49111)
Anti-Carbonic Anhydrase III antibody [2CA-4] (ab49111) at 1 µg/ml + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 29 kDa
Exposure time : 20 minutes
References for Anti-Carbonic Anhydrase III antibody [2CA-4] (ab49111)
ab49111 has not yet been referenced specifically in any publications.
Publishing research using ab49111? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"