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Anti-Carbonic Anhydrase III antibody (ab54913)

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Overview

Product name

Anti-Carbonic Anhydrase III antibody
See all Carbonic Anhydrase III products (5) ...

Description

Mouse monoclonal to Carbonic Anhydrase III

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length protein, corresponding to amino acids 1-261 of Human Carbonic Anhydrase III

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG1

Light chain type

kappa

  • IHC-P - Carbonic Anhydrase III antibody (ab54913)IHC-P - Carbonic Anhydrase III antibody (ab54913) image (enlarge)

  • Western blot - Carbonic Anhydrase III antibody (ab54913)Western blot - Carbonic Anhydrase III antibody (ab54913) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab54913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Reversible hydration of carbon dioxide.

Tissue specificity

Muscle specific.

Sequence similarities

Belongs to the alpha-carbonic anhydrase family.

Developmental stage

At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.

Post-translational
modifications

S-glutathionylated in hepatocytes under oxidative stress.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP07451 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CA III antibody
  • CA-III antibody
  • Ca3 antibody
  • CAH3_HUMAN antibody
  • CAIII antibody
  • Car3 antibody
  • Carbonate dehydratase III antibody
  • Carbonic anhydrase 3 antibody
  • Carbonic anhydrase III antibody
  • Carbonic anhydrase III muscle specific antibody
  • EC 4.2.1.1 antibody
see all

Anti-Carbonic Anhydrase III antibody images:

  IHC-P - Carbonic Anhydrase III antibody (ab54913)

IHC-P - Carbonic Anhydrase III antibody (ab54913)

Carbonic Anhydrase III antibody (ab54913) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human skeletal muscle.

  Western blot - Carbonic Anhydrase III antibody (ab54913)

Western blot - Carbonic Anhydrase III antibody (ab54913)

Carbonic Anhydrase III antibody (ab54913) at 1ug/lane + K-562 cell lysate at 25ug/lane.

References for Anti-Carbonic Anhydrase III antibody (ab54913)

This product has been referenced in:

  • Malm Cet al. Evaluation of 2-D DIGE for skeletal muscle: protocol and repeatability. Scand J Clin Lab Invest 68:793-800 (2008).Read more (PubMed: 18766954) »

See 1 publication for this product

Publishing research using ab54913? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"