Anti-Carbonic Anhydrase III antibody (ab54913)

Overview

• Product nameAnti-Carbonic Anhydrase III antibodySee all Carbonic Anhydrase III primary antibodies ...
• Description
  Mouse monoclonal to Carbonic Anhydrase III
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1-261 of Human Carbonic Anhydrase III

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cardiovascular
  • Hypoxia
  • Associated Proteins

  • Tags & Cell Markers
  • Cell Type Markers
  • Other Cell Types

  • Cell Biology
  • Other Antibodies
  • Other Antibodies

Applications

Target

• FunctionReversible hydration of carbon dioxide.
• Tissue specificityMuscle specific.
• Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
• Developmental stageAt 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
• Post-translational
  modificationsS-glutathionylated in hepatocytes under oxidative stress.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P07451 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 761 Human
  • Omim: 114750 Human
  • SwissProt: P07451 Human
  • Unigene: 82129 Human

• Alternative names
  CA III antibodyCA-III antibodyCa3 antibody

  CAH3_HUMAN antibodyCAIII antibodyCar3 antibodyCarbonate dehydratase III antibodyCarbonic anhydrase 3 antibodyCarbonic anhydrase III antibodyCarbonic anhydrase III muscle specific antibodyEC 4.2.1.1 antibody

  see all

Anti-Carbonic Anhydrase III antibody images

• 

  IHC-P - Carbonic Anhydrase III antibody (ab54913)
  Carbonic Anhydrase III antibody (ab54913) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human skeletal muscle.
• 

  Western blot - Carbonic Anhydrase III antibody (ab54913)
  
  
  Predicted band size : 29 kDa
  Carbonic Anhydrase III antibody (ab54913) at 1ug/lane + K-562 cell lysate at 25ug/lane.
• 

  Western blot - Carbonic Anhydrase III protein (ab50779)
  All lanes : Anti-Carbonic Anhydrase III antibody (ab54913) at 1 µg/ml
  
  Lane 1 : Carbonic Anhydrase III protein (ab50779) at 0.1 µg
  Lane 2 : Carbonic Anhydrase III protein (ab50779) at 0.01 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 90 seconds