Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464)
Key features and details
- Mouse monoclonal [10F7A8] to Carbonic Anhydrase 9/CA9 - N-terminal
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
-
Product name
Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal
See all Carbonic Anhydrase 9/CA9 primary antibodies -
Description
Mouse monoclonal [10F7A8] to Carbonic Anhydrase 9/CA9 - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Carbonic Anhydrase 9/CA9 aa 37-186 (N terminal). Expressed in E.coli. Predicted MW of fragment is 42 kDa.
Sequence:PQRL PRMQEDSPLG GGSSGEDDPL GEEDLPSEED SPREEDPPGE EDLPGEEDLP GEEDLPEVKP KSEEEGSLKL EDLPTVEAPG DPQEPQNNAH RDKEGDDQSH WRYGGDPPWP RVSPACAGRF QSPVDIRPQL AAFCPALRPL ELLGFQ
Database link: Q16790 -
Positive control
- Carbonic Anhydrase 9/CA9 recombinant protein, Carbonic Anhydrase IX (amino acids: 37-186)-hIgGFc transfected HEK293 cell lysate, A431 cell line lysate, SW620 cell line lysate, NTERA2 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% protein stabilizer consisting of amino acid =85% pH(1% water solution) =7.0~7.5 Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1 -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
10F7A8 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
|
|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
Tissue specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
Post-translational
modificationsAsn-346 bears high-mannose type glycan structures. -
Cellular localization
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
-
Alternative names
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
Images
-
All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution
Lane 1 : A431 cell line lysate
Lane 2 : SW620 cell line lysate
Predicted band size: 50 kDa -
All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution
Lane 1 : HEK293 cell lysate.
Lane 2 : Carbonic Anhydrase 9/CA9 (Amino acids: 37-186)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 50 kDa -
Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution + Carbonic Anhydrase 9/CA9 recombinant protein
Predicted band size: 50 kDa -
Flow cytometric analysis of NTERA2 cells labeling Carbonic Anhydrase 9/CA9 with ab181464 at 1/200 dilution (green) and negative control (red).
Datasheets and documents
-
Datasheet download
References (0)
ab181464 has not yet been referenced specifically in any publications.