Products:Cardiovascular >> Hypoxia >> Associated Proteins
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ab109840 |
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ab15086 has been referenced in 14 publications.
Publishing research using ab15086? Please let us know so that we can cite the reference in this datasheet
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Formalin fixed paraffin embedded renal carcinoma tissue, positively stained using Ab15086.
ab15086 at 1/500 staining dog gastric mucosa tissue sections by IHC-P. The tissue was formaldehyde fixed and a standard citrate buffer was used for anitgen retrieval. The tissue was blocked with 10% serum prior to incubation with the antibody for 15 hours. A biotinylated donkey anti-rabbit IgG (H + L) was used as the secondary.
This image is courtesy of an anonymous Abreview
All lanes : Anti-Carbonic Anhydrase IX antibody (ab15086) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 49.7 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
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