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Products:Cardiovascular >> Hypoxia >> Associated Proteins
Overview
Product name
Anti-Carbonic Anhydrase IX antibody
See all Carbonic Anhydrase IX products (7) ...
Description
Rabbit polyclonal to Carbonic Anhydrase IX
Tested applications
IHC-P, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide made to a sequence within residues 359-459 of the human protein (C-terminus), accession# Q16790
Positive control
Renal carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Hypoxia >> Associated Proteins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Carbonic Anhydrase IX antibody (ab15086)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Carbonic Anhydrase IX antibody (ab15086)
(enlarge)
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Western blot - Carbonic Anhydrase IX antibody (ab15086)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab15086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1000 - 1/2000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(Citrate Buffer (pH 6.0))
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20515941Slides were fixed in acetone for 20 minutes.)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with RND1 protein (His tag) (ab109840).
Target
Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Tissue specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Post-translational
modifications
Asn-346 bears high-mannose type glycan structures.
Cellular localization
Nucleus. Nucleus > nucleolus. Cell membrane. Cell projection > microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
Target information above from: UniProt accessionQ16790
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CA IX antibody
- CA-IX antibody
- CA9 antibody
see all
Database links
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
Anti-Carbonic Anhydrase IX antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Carbonic Anhydrase IX antibody (ab15086)
Formalin fixed paraffin embedded renal carcinoma tissue, positively stained using Ab15086.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Carbonic Anhydrase IX antibody (ab15086)
ab15086 at 1/500 staining dog gastric mucosa tissue sections by IHC-P. The tissue was formaldehyde fixed and a standard citrate buffer was used for anitgen retrieval. The tissue was blocked with 10% serum prior to incubation with the antibody for 15 hours. A biotinylated donkey anti-rabbit IgG (H + L) was used as the secondary.
This image is courtesy of an anonymous Abreview
Western blot - Carbonic Anhydrase IX antibody (ab15086)
All lanes : Anti-Carbonic Anhydrase IX antibody (ab15086) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 49.7 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
References for Anti-Carbonic Anhydrase IX antibody (ab15086)
This product has been referenced in:
- Brockton Net al. High stromal carbonic anhydrase IX expression is associated with decreased survival in P16-negative head-and-neck tumors. Int J Radiat Oncol Biol Phys 80:249-57 (2011). IHC-P; Human.Read more (PubMed: 21300470) »
- Ameri Ket al. Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype. Br J Cancer 102:561-9 (2010). WB; Human.Read more (PubMed: 20051957) »
See all 13 publications for this product
Publishing research using ab15086? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"