Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 9/CA9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Carbonic Anhydrase 9/CA9 antibody
See all Carbonic Anhydrase 9/CA9 primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 9/CA9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Carbonic Anhydrase 9/CA9 aa 1-168. The exact sequence is proprietary.
Database link: Q16790 -
Positive control
- HepG2 cell lysate, treated with 200µM CoCl2 for 24hr; Human cervical carcinoma tissue; CoCl2- treated A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
Tissue specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
Post-translational
modificationsAsn-346 bears high-mannose type glycan structures. -
Cellular localization
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
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Alternative names
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution.
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All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate, untreated
Lane 2 : HepG2 whole cell lysate, treated with 200 µM CoCl2 for 24 hr
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa10% SDS PAGE
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Immunofluorescent analysis of A431 cells labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution. A431 cells
were fixed in ice-cold MeOH for 5 min. Cells were treated with 200 μM CoCl2 (right) or untreated (left)
Green: Carbonic Anhydrase 9/CA9 protein showing membrane staining.
Blue: Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab184006 has been referenced in 12 publications.
- Bess SN et al. Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy. BMC Immunol 23:20 (2022). PubMed: 35461243
- Qorri B et al. Repositioning of Old Drugs for Novel Cancer Therapies: Continuous Therapeutic Perfusion of Aspirin and Oseltamivir Phosphate with Gemcitabine Treatment Disables Tumor Progression, Chemoresistance, and Metastases. Cancers (Basel) 14:N/A (2022). PubMed: 35892853
- Grgic I et al. Tumor Oxygenation by Myo-Inositol Trispyrophosphate Enhances Radiation Response. Int J Radiat Oncol Biol Phys 110:1222-1233 (2021). PubMed: 33587991
- Wang Q et al. Combined transcriptomic and lipidomic analysis reveals aberrant lipid metabolism in central nervous system hemangioblastomas. Sci Rep 11:1314 (2021). PubMed: 33446752
- Chen XJ et al. A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner. Angiogenesis 24:549-565 (2021). PubMed: 33484377