Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)

  Sandwich ELISA - Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)

Abcam's protein quantity microplate assays use the well-established sandwich ELISA format, whereby capture and detector antibodies are used to immobilize and then quantify a target protein or enzyme. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, a second monoclonal antibody, against a different epitope on the target, is added to the well. This detector antibody is either directly labeled with biotin, or a biotin-labeled goat anti-mouse secondary is added. Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.

  - Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)

Figure 1. Using this assay the acetylcholinesterase inhibitor tacrine, previosuly shown to co-crystalize in the active site of CES1, does not inhibit the activity of the enzyme which is in agreement with the crystalization study (Bencharit et al. Chem Biol 2003; 10(4) 341-9). Troglitazone a member of the thiazolidinediones class of antidiabetic drugs, was determined to inhihibit CES1 (IC50 3µM) while other members of this class had little/no effect (Fukami et al. Drug Metab Dispos 2010; 38(12):2173-8.