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MitoSciences (MS789)

Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)

Q&A (8)

Overview

  • Product nameCarboxylesterase 1 (CES1) Specific Activity Assay Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue
  • Assay typeEnzyme activity
  • Species reactivity
    Reacts with: Rat, Human
  • Product overview

    ab109717 (MS789) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate carboxylesterase 1 (CES1) from liver tissue or cell samples. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated CES1 is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

     

  • Notes

    All components are shipped cold. Store all components at 4°C.

  • Tested applicationsFunctional Studies more details

Properties

  • Alternative names
      ACATAcyl coenzyme A:cholesterol acyltransferaseBrain carboxylesterase hBr1
      CES1Cocaine carboxylesteraseEgasynHMSELiver carboxylesterase 1Monocyte/macrophage serine esteraseREHRetinyl ester hydrolaseSerine esterase 1TGHTriacylglycerol hydrolase
    see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab109717 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Functional Studies FuncS

      Carboxylesterase 1 (CES1) Specific Activity Assay Kit images

      • - Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)
        - Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)
        Figure 1. Using this assay the acetylcholinesterase inhibitor tacrine, previosuly shown to co-crystalize in the active site of CES1, does not inhibit the activity of the enzyme which is in agreement with the crystalization study (Bencharit et al. Chem Biol 2003; 10(4) 341-9). Troglitazone a member of the thiazolidinediones class of antidiabetic drugs, was determined to inhihibit CES1 (IC50 3µM) while other members of this class had little/no effect (Fukami et al. Drug Metab Dispos 2010; 38(12):2173-8.
      • Sandwich ELISA - Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)
        Sandwich ELISA - Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)
        Principle of Sandwich ELISA used in Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717). Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.

      Protocols

      References for Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)

      ab109717 has not yet been referenced specifically in any publications.

      Publishing research using ab109717 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab109717 Submit a Question for ab109717

      Displaying 1 - 8 of 8 results for Abreviews and Q&A

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                      Thank you for addressing this and getting me another kit!  My team and I really appreciate it!  Our order number was: xxxxx

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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      I purchased the CES1 Specific activity assay kit in June of this year. The product insert indicated that the guarantee is for 6 months from time of delivery. I ran the assay shortly after receipt and the ELISA worked well for undiluted blood samples (whic...

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      Thank you for your message and for providing this further information.

      I am sorry to hear that this product has not performed as expected. While the kit should have kept at these temperatures for this period it is possible that for some reaso...

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      Standard curve is shifted to the left compared to that shown in the protocol (the curve starts to fall after ˜120 ug/mL).

      Thank you very much for your call yesterday and for sending all of this data.
      The kit developers at MitoSciences have reviewed the data and agree that it looks good, and the top three points can be removed to improve the fit of the regression line. T...

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      Thank you for your reply. I am using a plate shaker at slower speeds for all incubations not involving plate reads. The only time the speed is higher is in between each interval of plate reads, first with the activity solution to assay activity and then w...

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      Thank you for your reply.

      I expect the assay will still give good results with your settings, but if you do see any unexpected data, please let me know and I will be happy to help you further.

      I know that you use a shaking speed of 400 RPM, but the lowest setting on our machine is 1020 RPM with an amplitude of 0.216" on the x-axis. Do you think this fater shaking speed will negatively impact my results?

      Thank you for your phone call.

      My colleague expressed concerns that since the speed of your shaker is so fast, you may see your samples spread too high up the side walls, sill out of the wells, or froth. If none of these are observed, then it is...

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      How should the 1x wash buffer and 1x incubation buffer bestored, and how long will they stable?
      Can the unused antibody strips be stored in the same packaging at 4C?
      For the plate shaking steps, how vigorously should the plate be shaked? How lon...

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      Thank you very much for your call this morning and for your patience while I've been in touch with the scientists in the MitoSciences lab regarding this kit.

      I went ahead and forwardedyour otherquestions as well, and here is the information that...

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      kit calls for reading at 402 nm. our reader is 360/40-only goes up to 400 nm. will I be able to use this kit?

      I looked at the spectrum - 405nm is the peak absorbance, but 360nm should work particularly with the wide window indicated. This assay will visibly turn yellow, so if there is any question about whether instrumentation is working correctly i...

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      The protocol says to measure OD at a "user determined time" for the endpoint assay. Do you have a recommendation for that time for HepG2 cells?

      Thank you for your inquiry. The protocol says to measure the wavelength at 402nm every minute for up to 1 hour for a kinetic assay. But if you would like to make an endpoint reading rather than kinetic, this can be done by observing the yellow color d...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"