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Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

MSCatalog No. MSZ04

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Overview

Product name

Anti-Carboxypeptidase Y antibody [10A5B5]
See all Carboxypeptidase Y products (4) ...

Description

Mouse monoclonal [10A5B5] to Carboxypeptidase Y

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Saccharomyces cerevisiae

Immunogen

Full length native protein (purified)

Positive control

Yeast membranes

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.

Clonality

Monoclonal

Clone number

10A5B5

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Signal Transduction >> Metabolism >> Amino Acids

  • Western blot - Carboxypeptidase Y antibody [10A5B5] (ab113685)Western blot - Carboxypeptidase Y antibody [10A5B5] (ab113685) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab113685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

  • ShowHide

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 20 µg/ml.

Target

Relevance

Carboxypeptidase Y is involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.

Cellular localization

Cytoplasmic

Alternative names

  • Carboxypeptidase Y antibody
  • Carboxypeptidase YSCY antibody
  • LBC1 antibody
  • MSZ04 antibody
  • PRC1 antibody
  • Vacuolar carboxypeptidase Y antibody
see all

Database links

Anti-Carboxypeptidase Y antibody [10A5B5] images:

  Western blot - Carboxypeptidase Y antibody [10A5B5] (ab113685)

Western blot - Carboxypeptidase Y antibody [10A5B5] (ab113685)

All lanes : Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

Lane 1 : Yeast whole cell lysate
Lane 2 : Yeast membrane preparation

Lysates/proteins at 15 µg per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 59 kDa

References for Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

This product has been referenced in:

  • Chen J  et al. Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy. PLoS One 7:e34024 (2012).Read more (PubMed: 22529904) »

See 1 publication for this product

Publishing research using ab113685? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"