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Carcino Embryonic Antigen (CEA) Human ELISA Kit (ab99992)

Submit an Abreview Q&A (6)

Overview

  • Product nameCarcino Embryonic Antigen (CEA) Human ELISA KitSee all Carcino Embryonic Antigen CEA kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.2 ng/ml
  • Range
    0.343 ng/ml - 250 ng/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116.7 93% - 136%
    Serum 145.6 135% - 156%
    Plasma 103.8 82% - 124%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's Carcino Embryonic Antigen CEA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Carcino Embryonic Antigen CEA in serum, plasma and cell culture supernatants.

     

    This assay employs an antibody specific for Human Carcino Embryonic Antigen CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and Carcino Embryonic Antigen CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Carcino Embryonic Antigen CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Carcino Embryonic Antigen CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details
  • PlatformMicroplate

Properties

  • RelevanceCarcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
    • Alternative names
        Carcinoembryonic antigen Carcinoembryonic antigen related cell adhesion molecule 5CD66e
        CD66e antigenCEACEACAM 5CEACAM5DKFZp781M2392Meconium antigen 100
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99992 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Carcino Embryonic Antigen (CEA) Human ELISA Kit images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99992.

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99992.

      • ELISA - Carcino Embryonic Antigen CEA Human ELISA Kit (ab99992)
        ELISA - Carcino Embryonic Antigen CEA Human ELISA Kit (ab99992)

      References for Carcino Embryonic Antigen (CEA) Human ELISA Kit (ab99992)

      ab99992 has not yet been referenced specifically in any publications.

      Publishing research using ab99992 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab99992 Submit a Question for ab99992

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

      10
      Votes: Highest First

      Can you tell me which antibody is used as a capture antibody for the CEA ELISA # ab99992 that you market?

      Thank you for your inquiry.

      The capture antibody in this kit is supplied by an outside lab and unfortunately they were not willing to provide me with any other details about it. They did want to know if you were interested in it to follow up on ...

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      Dear technical team,

      Our customer purchased ab99992 last November and she opened this kit today to test.
      And she confirmed there are 5μl of item G not 200μl so she would like to know if you have alternative item.
      Please let me k...

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      Thank you for your inquiry.

      I can confirm that the kit should contain 8 ul of HRP-Streptavidin Concentrate (Item G). Please tell your customer to spin down the vial. The kit was ordered in November 2011 and we did change the amount of HRP-Strept...

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      Sorry for bothering you again, but I was wondering if you happen to know if there is an anti-CEA antibody available in you company that is HRP-conjugated? In the report of a PhD student before me I read that she purchased anti-CEA coated with HRP from Abc...

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      Thank you for your new enquiry and continuous interest in our products.

      I have conducted a search for you and we have FITC and PE-conjugated antibodies but not HRP-linked primary antibody against CEA.

      However, we have two CEA ELISA kit...

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      is there any information regarding detection of mouse proteinwith this kit?

      Unfortunately there is no cross reactivity data available testing the Human CEA ELISA Kit ab99992 with mouse. If it helps though, the capture antibody is a mouse monoclonal and reacts with the protein determinant belonging to the “gold” epitope group 4. T...

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      I am interested in ab99992 but also want to compare it to a new technology I am developing and am wondering if I may also purchase the primary antibody used in that kit although it is not in your catalog.

      At the moment we do not sell kit components separately, this is because that we only produce enough reagents for use with the kit and do not have any spare to sell separately. We may in future add the antibody in its own right but for now it can only be ...

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      Dear technical team, Our customer would like to purchase CEA ELISA kit and alpha Fetoprotein ELISA Kit. She has searched ab108631, ab108838, ab108635, ab99992 and asked well plate type.   Is it strip type or one plate type?  

      Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I hop...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"