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Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)

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Overview

Product name

Anti-Carcino Embryonic Antigen CEA antibody [26/3/13]
See all Carcino Embryonic Antigen CEA products (22) ...

Description

Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA

Specificity

This antibody recognises exclusively CEACAM 5 transiently expressed on the cell surface of transfected BOSC cells. It can be used to distinguish CEACAM 5 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules, namely CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM7), CEACAM 6 (NCA/CD66c), CEACAM 7 (CGM2), CEACAM 8 (CGM6/CD66b)and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members. This antibody was included and characterized in the studies from the VIth Leucocyte Typing Workshop (Grunert F et al. 1994)

Tested applications

Sandwich ELISA, ELISA, Flow Cyt, WB, IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length native protein (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Grunert F, et al. 1985)

General notes

Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

26/3/13

Isotype

IgG1

Research areas

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

  • Sandwich ELISA - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)Sandwich ELISA - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.

Tissue specificity

Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Sequence similarities

Belongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains.

Post-translational
modifications

Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP06731 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Carcinoembryonic antigen antibody
  • Carcinoembryonic antigen related cell adhesion molecule 5 antibody
  • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
  • CD66e antibody
  • CD66e antigen antibody
  • CEA antibody
  • CEA antibody
  • CEACAM 5 antibody
  • CEACAM5 antibody
  • CEAM5_HUMAN antibody
  • DKFZp781M2392 antibody
  • Meconium antigen 100 antibody
  • OTTHUMP00000199032 antibody
  • OTTHUMP00000199033 antibody
  • OTTHUMP00000199034 antibody
see all

Database links

Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] images:

  Sandwich ELISA - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)

Sandwich ELISA - Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)

Standard Curve for Carcino Embryonic Antigen CEA dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA (ab4451) at 0.2ug/ml and Detector Antibody Rabbit polyclonal to Carcino Embryonic Antigen CEA (ab15987) at 0.5ug/ml

Protocols

  • Protocol Booklet

    • References for Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)

    This product has been referenced in:

    • Grunert F CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules. Leukocyte Typing VI: White cell Differen. Antigens :1012-1025 (1996).Read more (PubMed: ) »

    See 1 publication for this product

    Publishing research using ab4451? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"