Anti-Carcino Embryonic Antigen CEA antibody (ab15987)

Thank you for your kind help. So after this conversation, I can understand that I can use your HRP kit for the labeling for the secondary antibody. In that case there is no need to use tertiary antibody.

ANSWER:

Thank you for your email.
Yes, that is correct. Alternatively, you can just use a secondary antibody that has already been conjugated with HRP. We carry many in our catalog. Please let me know if you need help selecting one.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for your kind reply. Can your HRP conjugation kit (ab102891) be able to solve the above problems which may arise during the use of other company's HRP kit?

ANSWER:

The conjugation kit ab102891 is compatible with antibody formulations that contain up to 1% BSA, even though the BSA is in 10-fold excess to the antibody (10mg/ml BSA compared to 1 mg/ml IgG for ab71720). Some of the BSA will itself will be conjugated, affecting efficient conjugation of the IgG, so an antibody without BSA would be better, such as anti-PSMA ab19071.

However, even with 1% BSA, much of the IgG in ab71720 can be conjugated to HRP using the HRP kit ab102891, and will be useful for subsequent assays. I do not know if that is the case for the other company's conjugation kit.

Thank you very much for your kind reply. Is it necessary to use 3rd labeled antibody?
We have HRP labeling kit-SH from another company. So, is it possible to label the ab15987 (for CEA) and ab71720 (for PSMA) instead of using the 3rd labeled antibody (e.g.ab97080 or ab97080)?
Also can we label the primary antibody by the same kit?

ANSWER:

I think the CEA antibody formulation will be compatible with the other company's labeling kit but you should contact them and send them the buffer components. Here are the components of the ab15987 buffer:

Preservative: 15mM Sodium Azide Constituents: 0.1M Sodium chloride, pH 7.2

The concentration of IgG for our current lot of ab15987 is 2.0 mg/ml. There is no protein carrier such as BSA.

The PSMA antibody formulation (ab97080) may be more problematic, as it contains BSA, which may interfere with conjugation of the HRP to the immunoglobulin. The buffer components are:

Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4

The IgG concentration of ab97080 is 1.0 mg/ml.

Again, the other company will be able to tell you if these components are compatible with their conjugation kit. You may need to remove the BSA, in which case I recommend our purification product, ab102784.

http://www.abcam.com/index.html?datasheet=102784

We also have an HRP conjugation kit, if you are interested. The catalogue number is ab102891.

http://www.abcam.com/index.html?datasheet=102891

I hope this is helpful. If you have any questions, please contact us.

Thanks for the reply.

Any one of these biomarkers is good for me.

CA 15.3

TRU-QUANT

CA 27.29

CA125

CEA (carcinoembryonic antigen)



thanks

ANSWER:

Thank you for your resposne.

As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

As ELISA is the closest application, I am pleased to recommend some antibodies suitable for this applications, however these will not be covered by our guarantee for electrical biosensors.

CA 15.3 - none

TRU-QUANT - none

CA 27.29 - none

CA125 - ab10033, ab10029, ab19551

CEA (carcinoembryonic antigen) - ab15987, ab4451,



If you need any further assistance in the future, please do not hesitate to contact me.

Dear technical team,

Our customer would like to purchase ab4451 and ab15987 for sELISA and will apply ab742 as a standard.
I checked ab742 doesn’t guarantee for ELISA but let me know if it is possible.

Best regards,

ANSWER:

Thank you for your enquiry.

I can confirm that ab4451 and ab15987 have been tested and guarantee for use in sELISA together according to the datasheets. Theab742 Carcino Embryonic Antigen CEA protein is full length native protein so should be suitable as a standard for use with this antibodies.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.