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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-Carcino Embryonic Antigen CEA antibody
See all Carcino Embryonic Antigen CEA products (21) ...
Description
Rabbit polyclonal to Carcino Embryonic Antigen CEA
Specificity
Ab15987 reacts with Carcino Embryonic Antigen (CEA) and CEA like proteins, for example, CEACAM1 and CEACAM6.
Tested applications
Sandwich ELISA, Immunoelectrophoresis, IHC-Fr, IHC-P, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Human Carcino Embryonic Antigen isolated from hepatic metastasis of colon adenocarcinoma.
Positive control
Perchloric acid extract of hepatic metastasis of colon adenocarcinoma. Epithelial cells in colon, bronchial and alveolar epithelium, and alveolar macrophages in lung, polymorphonuclear leukocytes and macrophages in spleen, and the superficial epithelial layer and parietal cells in stomach. In liver, predominantly bilary canaliculi are labelled.
General notes
Cells labelled by the antibody display a cytoplasmic staining pattern. In normal colon, the luminal border as well as the basal part of epithelial cells are strongly labelled. In colonic adenocarcinoma, positive staining is localized diffusely in the cytoplasm and in the gland lumina.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.1M Sodium chloride, pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Trace amounts of contaminating antibodies to human plasma proteins have been removed by solid phase absorption. Absorption has also been performed with blood group antigens A and B.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA (ab4451). (For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab4451 as Capture.)
Ie: Use at an assay dependent dilution. (Crossed. Only the CEA and a CEA like protein precipitation arches appear when using 12.5 µL antibody per square. No reaction is seen with 2 µL normal human plasma.)
IHC-Fr: Use at an assay dependent dilution.
IHC-P: 1/200 - 1/400.Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ELISA: Use at an assay dependent dilution. (In double antibody sandwich ELISA, no reaction is observed with normal human plasma stripped of CEA.)
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Tissue specificity
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains.
Post-translational
modifications
Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP06731
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Carcinoembryonic antigen antibody
- Carcinoembryonic antigen related cell adhesion molecule 5 antibody
- Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
see all
Database links
- Entrez Gene: 1048 Human
- Omim: 114890 Human
- SwissProt: P06731 Human
- Unigene: 709196 Human
Anti-Carcino Embryonic Antigen CEA antibody images:
Sandwich ELISA - Carcino Embryonic Antigen CEA antibody (ab15987)
Standard Curve for Carcino Embryonic Antigen CEA dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA (ab4451) at 0.2ug/ml and Detector Antibody Rabbit polyclonal to Carcino Embryonic Antigen CEA (ab15987) at 0.5ug/ml
Immunocytochemistry/ Immunofluorescence - Carcino Embryonic Antigen CEA antibody (ab15987)
ICC/IF image of ab15987 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15987, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Carcino Embryonic Antigen CEA antibody (ab15987)
This product has been referenced in:
- Fernando S & Fletcher BS Targeting tumor endothelial marker 8 in the tumor vasculature of colorectal carcinomas in mice. Cancer Res 69:5126-32 (2009). IHC-P; Human.Read more (PubMed: 19528090) »
- Hallermalm Ket al. Pre-clinical evaluation of a CEA DNA prime/protein boost vaccination strategy against colorectal cancer. Scand J Immunol 66:43-51 (2007).Read more (PubMed: 17587345) »
See all 2 publications for this product
Publishing research using ab15987? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"